Localization of all four ZnT zinc transporters inDictyosteliumand impact of ZntA and B knockout on bacteria killing
| العنوان: | Localization of all four ZnT zinc transporters inDictyosteliumand impact of ZntA and B knockout on bacteria killing |
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| المؤلفون: | Vera Kalinina, Caroline Barisch, Louise H. Lefrançois, Ana Teresa López-Jiménez, Joddy Shem Appiah, Thierry Soldati |
| المصدر: | Journal of Cell Science, Vol. 131, No 23 (2018) |
| بيانات النشر: | The Company of Biologists, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, biology, Endosome, Phagocytosis, Phagosome acidification, chemistry.chemical_element, Cell Biology, Zinc, Vacuole, biology.organism_classification, Dictyostelium discoideum, Cell biology, 03 medical and health sciences, 030104 developmental biology, chemistry, ddc:540, Efflux, Phagosome |
| الوصف: | Professional phagocytes have developed an extensive repertoire of autonomous immunity strategies to ensure killing of bacteria. Besides phagosome acidification and the generation of reactive oxygen species, deprivation of nutrients and the lumenal accumulation of toxic metals are essential to kill ingested bacteria or inhibit growth of intracellular pathogens. We use the soil amoeba Dictyostelium discoideum, a professional phagocyte that digests bacteria for nutritional purposes, to decipher the role of zinc poisoning during phagocytosis of non-pathogenic bacteria and visualize the temporal and spatial dynamics of compartmentalized, free zinc using fluorescent probes. Immediately after particle uptake, zinc is delivered to phagosomes by fusion with “zincosomes” of endosomal origin, but also by the action of one or more zinc transporters. We localize the four Dictyostelium ZnT transporters to endosomes, the contractile vacuole and the Golgi apparatus, and study the impact of znt knockouts on zinc homeostasis. Finally, we show that zinc is delivered into the lumen of Mycobacterium smegmatis-containing vacuoles, and that Escherichia coli deficient in the zinc efflux P1B-type ATPase ZntA is killed faster than wild type bacteria. |
| تدمد: | 1477-9137 0021-9533 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::515d5f5f85e37c864317b810562278e9 https://doi.org/10.1242/jcs.222000 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....515d5f5f85e37c864317b810562278e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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