Protective activities of distinct omega-3 enriched oils are linked to their ability to upregulate specialized pro-resolving mediators
| العنوان: | Protective activities of distinct omega-3 enriched oils are linked to their ability to upregulate specialized pro-resolving mediators |
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| المؤلفون: | Romain A. Colas, Agua Sobrino, Mary Walker, Jesmond Dalli |
| المصدر: | PLOS ONE PLoS ONE PLoS ONE, Vol 15, Iss 12, p e0242543 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Apolipoprotein E, Male, Mice, Knockout, ApoE, Gene Expression, 030204 cardiovascular system & hematology, Pharmacology, Biochemistry, Vascular Medicine, Mice, White Blood Cells, 0302 clinical medicine, Medical Conditions, Animal Cells, Gene expression, Medicine and Health Sciences, Arachidonate 15-Lipoxygenase, Lipoxygenase Inhibitors, Immune Response, Principal Component Analysis, Multidisciplinary, Chemistry, Eukaryota, Plants, Lipids, Lipoproteins, LDL, Lipoxins, Cell Processes, Medicine, Female, medicine.symptom, Cellular Types, Research Article, Leukotrienes, Algae, Phagocytosis, Science, Immune Cells, Inflammatory Diseases, Primary Cell Culture, Immunology, Inflammation, 03 medical and health sciences, Lipid Mediators, Apolipoproteins E, Signs and Symptoms, Downregulation and upregulation, Fatty Acids, Omega-3, medicine, Animals, Humans, Arachidonate 5-Lipoxygenase, Blood Cells, Macrophages, fungi, Organisms, Biology and Life Sciences, Lipid signaling, Cell Biology, Atherosclerosis, In vitro, 030104 developmental biology, Cell culture, Diet, Western, Dietary Supplements, Prostaglandins, Clinical Medicine, Oils |
| الوصف: | Clinical studies using a range of omega-3 supplements have yielded conflicting results on their efficacy to control inflammation. Omega-3 fatty acids are substrate for the formation of potent immune-protective mediators, termed as specialized pro-resolving mediators (SPM). Herein, we investigated whether observed differences in the potencies of distinct omega-3 supplements were linked with their ability to upregulate SPM formation. Using lipid mediator profiling we found that four commercially available supplements conferred a unique SPM signature profile to human macrophages, with the overall increases in SPM concentrations being different between the four supplements. These increases in SPM concentrations were linked with an upregulation of macrophage phagocytosis and a decreased uptake of oxidized low-density lipoproteins. Pharmacological inhibition of two key SPM biosynthetic enzymes 5-Lipoxygenase or 15-Lipoxygenase reversed the macrophage-directed actions of each of the omega-3 supplements. Furthermore, administration of the two supplements that most potently upregulated macrophage SPM formation and reprogrammed their responses in vitro, to APOE-/- mice fed a western diet, increased plasma SPM concentrations and reduced vascular inflammation. Together these findings support the utility of SPM as potential prognostic markers in determining the utility of a given supplement to regulate macrophage responses and inflammation. |
| تدمد: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0242543 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5165e1943bf439897bf8b97869c08ace |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5165e1943bf439897bf8b97869c08ace |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0242543 |