Antibiotic contamination of water and food is a particularly important environmental worldwide problem. Antibiotics are found in water, soil, and food, which negatively affects both the environment and the health of the entire world’s population. Therefore, it is important to control the content of antimicrobials. To solve this problem, scientists are developing new and improving existing methods for these preparation determination. The paper considers the possibility of applying the technique of high-performance liquid chromatography with mass spectrometric detection for the determination of nitroimidazole group antibiotics in meat raw materials. The method was evaluated in terms of the main characteristics taken into account during validation: accuracy, reproducibility, linearity, and specificity. Studies conducted on pork muscle tissue samples showed the absence of antibiotics of this group in all samples, which meets the requirements of regulatory documents.
