The effect of differentiation and TGFβ on mitochondrial respiration and mitochondrial enzyme abundance in cultured primary human skeletal muscle cells
| العنوان: | The effect of differentiation and TGFβ on mitochondrial respiration and mitochondrial enzyme abundance in cultured primary human skeletal muscle cells |
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| المؤلفون: | Lisa Kappler, Christoph Hoffmann, Hans-Ulrich Häring, Selina Höckele, Martin Hrabĕ de Angelis, Cora Weigert |
| المصدر: | Scientific Reports Sci. Rep. 8:737 (2018) Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cellular respiration, Cellular differentiation, Cell Respiration, lcsh:Medicine, Oxidative phosphorylation, Mitochondrion, Article, Electron Transport, Electron Transport Complex IV, 03 medical and health sciences, 0302 clinical medicine, Transforming Growth Factor beta, medicine, Myocyte, Humans, lcsh:Science, Muscle, Skeletal, Cells, Cultured, Muscle Cells, Multidisciplinary, Myogenesis, Muscle cell differentiation, Chemistry, lcsh:R, Skeletal muscle, Cell Differentiation, Middle Aged, Cell biology, Mitochondria, Oxygen, 030104 developmental biology, medicine.anatomical_structure, lcsh:Q, 030217 neurology & neurosurgery |
| الوصف: | Measuring mitochondrial respiration in cultured cells is a valuable tool to investigate the influence of physiological and disease-related factors on cellular metabolism; however, the details of the experimental workflow greatly influence the informative value of the results. Working with primary cells and cell types capable of differentiation can be particularly challenging. We present a streamlined workflow optimised for investigation of primary human skeletal muscle cells. We applied the workflow to differentiated and undifferentiated cells and we investigated the effect of TGFβ1 treatment. Differentiation of myoblasts to myotubes increased mitochondrial respiration and abundance of mitochondrial enzymes and mitochondrial marker proteins. Differentiation also induced qualitative changes in mitochondrial protein composition and respiration. TGFβ1 reduced complex IV protein MTCO1 abundance in both myoblasts and myotubes. In myoblasts, spare electron transport system (ETS) capacity was reduced due to a reduction in maximal oxygen consumption. In TGFβ1-treated myotubes, the reduction in spare ETS capacity is mainly a consequence of increased oxidative phosphorylation capacity and complex III protein UQCRC2. Taken together, our data shows that it is important to monitor muscle cell differentiation when mitochondrial function is studied. Our workflow is not only sensitive enough to detect physiological-sized differences, but also adequate to form mechanistic hypotheses. |
| وصف الملف: | application/pdf |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5184bbe25271ff6df76f6219c95590dc https://pubmed.ncbi.nlm.nih.gov/29335583 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5184bbe25271ff6df76f6219c95590dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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