RNase E-dependent degradation of tnaA mRNA encoding tryptophanase is prerequisite for the induction of acid resistance in Escherichia coli
| العنوان: | RNase E-dependent degradation of tnaA mRNA encoding tryptophanase is prerequisite for the induction of acid resistance in Escherichia coli |
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| المؤلفون: | Takeshi Kanda, Yu Kanesaki, Masaaki Wachi, Noritaka Iwai, Hirofumi Yoshikawa, Genta Abiko |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Indoles, RNase P, 030106 microbiology, Glutamate decarboxylase, Mutant, lcsh:Medicine, medicine.disease_cause, Article, Gastric Acid, 03 medical and health sciences, Endoribonucleases, Escherichia coli, medicine, RNA, Messenger, lcsh:Science, Indole test, Messenger RNA, Multidisciplinary, Glutamate Decarboxylase, Chemistry, Escherichia coli Proteins, Hydrolysis, lcsh:R, Tryptophanase, Bacteriology, Molecular biology, Culture Media, 030104 developmental biology, Enzyme Induction, bacteria, lcsh:Q, Pathogens, Bacterial outer membrane |
| الوصف: | Acid-resistance systems are essential for pathogenic Escherichia coli to survive in the strongly acidic environment of the human stomach (pH tolC mutant lacking the TolC outer membrane channel was defective in GAD induction. Here, we show that indole, a substrate of TolC-dependent efflux pumps and produced by the tryptophanase encoded by the tnaA gene, negatively regulates GAD expression. GAD expression was restored by deleting tnaA in the tolC mutant; in wild-type E. coli, it was suppressed by adding indole to the growth medium. RNA-sequencing revealed that tnaA mRNA levels drastically decreased upon exposure to moderately acidic conditions (pH 5.5). This decrease was suppressed by RNase E deficiency. Collectively, our results demonstrate that the RNase E-dependent degradation of tnaA mRNA is accelerated upon acid exposure, which decreases intracellular indole concentrations and triggers GAD induction. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51b0160ce0ffab118926f22927a2b4d1 https://doi.org/10.1038/s41598-020-63981-x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....51b0160ce0ffab118926f22927a2b4d1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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