Detection of nasopharyngeal carcinoma using surface-enhanced laser desorption and ionization mass spectrometry profiles of the serum proteome
| العنوان: | Detection of nasopharyngeal carcinoma using surface-enhanced laser desorption and ionization mass spectrometry profiles of the serum proteome |
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| المؤلفون: | Qiu Yan Chen, Chang Qing Zhang, Jie Kai Yu, Dan Xie, Chao Nan Qian, Ning Wei Li, Yan Qun Xiang, Su Mei Cao, Xun Hu, Xiang Guo |
| المصدر: | Chinese Journal of Cancer. 29:721-728 |
| بيانات النشر: | Editorial Office of Chinese Journal of Cancer, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Adult, Male, Proteomics, Nasopharyngeal neoplasm, Antibodies, Viral, Mass spectrometry, Sensitivity and Specificity, Antigen, Biomarkers, Tumor, otorhinolaryngologic diseases, Humans, Medicine, Ionization mass spectrometry, Antigens, Viral, Aged, biology, business.industry, Reproducibility of Results, Nasopharyngeal Neoplasms, Middle Aged, medicine.disease, Molecular biology, Neoplasm Proteins, Surface-enhanced laser desorption/ionization, stomatognathic diseases, Oncology, Nasopharyngeal carcinoma, Serum proteome, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, biology.protein, Capsid Proteins, Female, Antibody, business, Algorithms |
| الوصف: | BACKGROUND AND OBJECTIVE Early diagnosis of nasopharyngeal carcinoma (NPC) is difficult due to the insufficient specificity of the conventional examination method. This study was to investigate potential and consistent biomarkers for NPC, particularly for early detection of NPC. METHODS A proteomic pattern was identified in a training set (134 NPC patients and 73 control individuals) using the surface-enhanced laser desorption and ionization-mass spectrometry (SELDI-MS), and used to screen the test set (44 NPC patients and 25 control individuals) to determine the screening accuracy. To confirm the accuracy, it was used to test another group of 52 NPC patients and 32 healthy individuals at 6 months later. RESULTS Eight proteomic biomarkers with top-scored peak mass/charge ratios (m/z) of 8605 Da, 5320 Da, 5355 Da, 5380 Da, 5336 Da, 2791 Da, 7154 Da, and 9366 Da were selected as the potential biomarkers of NPC with a sensitivity of 90.9% (40/44) and a specificity of 92.0% (23/25). The performance was better than the current diagnostic method by using the Epstein-Barr virus (EBV) capsid antigen IgA antibodies (VCA/IgA). Similar sensitivity (88.5%) and specificity (90.6%) were achieved in another group of 84 samples. CONCLUSION SELDI-MS profiling might be a potential tool to identify patients with NPC, particularly at early clinical stages. |
| تدمد: | 1944-446X 1000-467X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51cecdd2ac3f0f1aabb716cefde21de1 https://doi.org/10.5732/cjc.010.10037 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....51cecdd2ac3f0f1aabb716cefde21de1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1944446X 1000467X |
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