Quantitative Study of the Chiral Organization of the Phage Genome Induced by the Packaging Motor
| العنوان: | Quantitative Study of the Chiral Organization of the Phage Genome Induced by the Packaging Motor |
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| المؤلفون: | Zihao Zhu, Javier Arsuaga, Carme Calderer, Brian Cruz, Mariel Vazquez |
| المصدر: | Biophysical Journal Biophysical journal, vol 118, iss 9 |
| بيانات النشر: | The Biophysical Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Biophysics, Genome, Viral, Rotation, Genome, Quantitative Biology::Subcellular Processes, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Capsid, DNA Packaging, Molecular motor, Genetics, Bacteriophages, Kinetic Monte Carlo, Viral, 030304 developmental biology, Writhe, Physics, 0303 health sciences, Quantitative Biology::Biomolecules, Human Genome, DNA, Articles, Biological Sciences, Quantitative Biology::Genomics, chemistry, Physical Sciences, Chemical Sciences, DNA, Viral, Brownian dynamics, Generic health relevance, Biological system, 030217 neurology & neurosurgery |
| الوصف: | Molecular motors that translocate DNA are ubiquitous in nature. During morphogenesis of double-stranded DNA bacteriophages, a molecular motor drives the viral genome inside a protein capsid. Several models have been proposed for the three-dimensional geometry of the packaged genome, but very little is known of the signature of the molecular packaging motor. For instance, biophysical experiments show that in some systems, DNA rotates during the packaging reaction, but most current biophysical models fail to incorporate this property. Furthermore, studies including rotation mechanisms have reached contradictory conclusions. In this study, we compare the geometrical signatures imposed by different possible mechanisms for the packaging motors: rotation, revolution, and rotation with revolution. We used a previously proposed kinetic Monte Carlo model of the motor, combined with Brownian dynamics simulations of DNA to simulate deterministic and stochastic motor models. We find that rotation is necessary for the accumulation of DNA writhe and for the chiral organization of the genome. We observe that although in the initial steps of the packaging reaction, the torsional strain of the genome is released by rotation of the molecule, in the later stages, it is released by the accumulation of writhe. We suggest that the molecular motor plays a key role in determining the final structure of the encapsidated genome in bacteriophages. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1542-0086 0006-3495 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51cf40ee623c13f6535ddcd11f4ff46b http://europepmc.org/articles/PMC7203069 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....51cf40ee623c13f6535ddcd11f4ff46b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15420086 00063495 |
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