Primary dissociated cultures of human brainstem cells: a useful tool for their characterization and neuroprotection study
| العنوان: | Primary dissociated cultures of human brainstem cells: a useful tool for their characterization and neuroprotection study |
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| المؤلفون: | Marie-Christine Calvet, Alain Privat, Jean-Marc Kamenka, Christine Levallois, Didier Petite |
| المصدر: | Cell Biology and Toxicology. 11:155-160 |
| بيانات النشر: | Springer Science and Business Media LLC, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Neurons, Glial fibrillary acidic protein, Health, Toxicology and Mutagenesis, Cell Culture Techniques, Cell Biology, Biology, Toxicology, Immunohistochemistry, Molecular biology, Neuroprotection, Rhombencephalon, Fetus, Biochemistry, Cell culture, Dopamine, biology.protein, medicine, Catecholamine, Humans, Locus coeruleus, Serotonin, Raphe nuclei, Cells, Cultured, Brain Stem, medicine.drug |
| الوصف: | Dissociated cell cultures were prepared from brainstems of 5- to 10-week-old human fetuses. Catecholamine- as well as indolamine-containing cells were visualized using respectively dopamine (DA), noradrenaline (NA) and serotonin (5HT) as immunocytochemical markers. NA-, DA-, and 5HT-stained cells were characterized in the rhombencephalic cultures, representing respectively the fetal localization of the locus coeruleus and raphe nuclei. DA-stained cells were characterized in the mesencephalic cultures; these DA-cells originating from the substantia nigra presented morphological aspects different from the DA-rhombencephalic cells. Two types of GABA neurons and glial cells presenting glial fibrillary acidic protein (GFA-P) reactivity were also found in all the cultures. Two non-competitive N-methyl-D-aspartate antagonists, 1-[1-(2-thienyl)cyclohexyl]piperidine (TCP) and cis-Pip/Me 1-[1-(2-thienyl)-2-methylcyclohexyl]piperidine (GK11) in enantiomeric form (-), have been investigated for survival on rhombencephalic cultured cells. The number of 5HT-cells was found to be greater in the treated cultures than in the control ones. This in vitro system appears to be a useful tool for the investigation of the development of central nervous system (CNS) cells as well as the study of neuroprotection. |
| تدمد: | 1573-6822 0742-2091 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52576459e3baf1bd943f3693e0cbc577 https://doi.org/10.1007/bf00756517 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....52576459e3baf1bd943f3693e0cbc577 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15736822 07422091 |
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