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Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes

التفاصيل البيبلوغرافية
العنوان: Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes
المؤلفون: Tetsuya, Harada, Yuki, Wakabayashi, Kazuko, Seto, Kenichi, Lee, Sunao, Iyoda, Kentaro, Kawatsu
المصدر: Diagnostic Microbiology and Infectious Disease. 105:115874
بيانات النشر: Elsevier BV, 2023.
سنة النشر: 2023
مصطلحات موضوعية: Microbiology (medical), Infectious Diseases, General Medicine
الوصف: To develop subtyping methods for Shiga toxin (Stx)1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g genes for epidemiological analyses of Shiga toxin-producing Escherichia coli (STEC), we developed 10 simplex real-time polymerase chain reaction (PCR) assays with reference to 284 valid stx sequences and evaluated their specificity and quantitative accuracy using STEC and non-STEC isolates and recombinant plasmids, respectively. Three stx
تدمد: 0732-8893
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5271ae8f0d2961e067557cde060bd62b
https://doi.org/10.1016/j.diagmicrobio.2022.115874
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....5271ae8f0d2961e067557cde060bd62b
قاعدة البيانات: OpenAIRE
الوصف
تدمد:07328893