Stx1 and Stx2 subtyping and antimicrobial resistance in Shiga toxin-producing Escherichia coli (STEC) isolates from cattle and sheep feces in the Southeastern region of the State of Goiás, Brazil
| العنوان: | Stx1 and Stx2 subtyping and antimicrobial resistance in Shiga toxin-producing Escherichia coli (STEC) isolates from cattle and sheep feces in the Southeastern region of the State of Goiás, Brazil |
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| المؤلفون: | Angélica Franco de Oliveira, C. N. Moreira, Nayara Carvalho Barbosa, Ângela Vitalina Barbosa de Assis Silveira, Benner G. Alves, Marcos Roberto Alves Ferreira, Ariel Eurides Stella, Bruna Ribeiro Arrais |
| المصدر: | Pesquisa Veterinária Brasileira v.41 2021 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Volume: 41, Article number: e06747, Published: 05 JUL 2021 Pesquisa Veterinária Brasileira, Vol 41 (2021) |
| بيانات النشر: | FapUNIFESP (SciELO), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | resistência antimicrobiana, subtyping, Veterinary medicine, Antibiotics, virulence factors, ruminantes, medicine.disease_cause, 0403 veterinary science, fluids and secretions, saúde pública, STX2, SF600-1100, fatores de virulência, 0303 health sciences, toxina Shiga, public health, bovinos, Shiga toxin, 04 agricultural and veterinary sciences, Subtyping, STEC, Brazil, sheep, 040301 veterinary sciences, medicine.drug_class, multidrug-resistant, ovinos, Biology, Subtipagem, Microbiology, 03 medical and health sciences, Antibiotic resistance, Escherichia coli, medicine, antimicrobial resistance, fezes, segurança alimentar, Feces, General Veterinary, 030306 microbiology, Brasil, food security, Multiple drug resistance, feces, cattle, ruminants, biology.protein, mutidroga resitentes, Stx1, Stx2 |
| الوصف: | The present study was aimed at subtyping of Stx1 and Stx2 genes and characterization of antimicrobial resistance in 106 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and sheep feces. PCR was used to determine the subtypes, and the disk-diffusion method was used to evaluate the antimicrobial resistance. Ten antibiotics from five different classes were tested. Among the isolates of bovine origin, two subtypes of Stx1 (Stx1a and Stx1c), and four subtypes of Stx2 (Stx2a, Stx2b, Stx2c, and Stx2d) were identified. In isolates of sheep origin, two subtypes of Stx1 (Stx1a and Stx1c), and four subtypes of Stx2 (Stx2a, Stx2b, Stx2c, and Stx2 g) were identified. The results obtained suggest the presence of high diversity in Stx1 and Stx2 genes. Further, 96.6% (57/59) of bovine fecal strains and 89.4% (42/47) of sheep fecal strains showed resistance to at least one tested antibiotic. In both animal species, most strains were multidrug-resistant (MDR) (67.8% in cattle and 59.6% in sheep), with no significant difference between host animals. Adult animals were eight times more likely to have STEC with greater pathogenic potential. STEC with the highest pathogenic potential were three times more likely to be multidrug-resistant than STEC with the lowest pathogenic potential. The data reported in this study suggests the occurrence of strains with high potential pathogenicity in the region studied. Therefore, the ruminants of this region are carriers of strains that can cause infections in humans. RESUMO: O presente estudo teve como objetivo subtipar os genes Stx1 e Stx2 e caracterizar a resistência antimicrobiana em 106 isolados de Escherichia coli produtoras de toxinas Shiga (STEC) isoladas de fezes de bovinos e ovinos. A PCR foi utilizada para determinar os subtipos e o método de difusão em disco foi utilizado para avaliar a resistência antimicrobiana. Dez antibióticos de cinco classes diferentes foram testados. Entre os isolados de origem bovina, foram identificados dois subtipos de Stx1 (Stx1a e Stx1c) e quatro subtipos de Stx2 (Stx2a, Stx2b, Stx2c e Stx2d). Nos isolados de origem ovina, foram identificados dois subtipos de Stx1 (Stx1a e Stx1c) e quatro subtipos de Stx2 (Stx2a, Stx2b, Stx2c e Stx2g). Os resultados obtidos sugerem a presença de alta variabilidade nos genes Stx1 e Stx2. Além disso, 96,6% (57/59) dos isolados fecais de bovinos e 89,4% (42/47) dos isolados de ovinos mostraram resistência a pelo menos um antibiótico testado. Em ambas as espécies animais, a maioria das cepas foi multirresistente (MDR) (67,8% em bovinos e 59,6% em ovinos), sem diferença significativa entre as espécies animais do reservatório. Os animais adultos tiveram oito vezes mais chances de apresentar STEC com maior potencial patogênico. STEC com o maior potencial patogênico teve três vezes mais chances de ser multirresistente do que o STEC com o menor potencial patogênico. Os dados relatados neste estudo sugerem a ocorrência de cepas com alto potencial de patogenicidade na região estudada. Portanto, os ruminantes dessa região são hospedeiros de isolados que podem causar infecções em humanos. |
| وصف الملف: | text/html |
| تدمد: | 1678-5150 0100-736X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5399313c0c3f814b95d03e154c638ca7 https://doi.org/10.1590/1678-5150-pvb-6747 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5399313c0c3f814b95d03e154c638ca7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16785150 0100736X |
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