Quantification of Genital Human Immunodeficiency Virus Type 1 (HIV-1) DNA in Specimens from Women with Low Plasma HIV-1 RNA Levels Typical of HIV-1 Nontransmitters
| العنوان: | Quantification of Genital Human Immunodeficiency Virus Type 1 (HIV-1) DNA in Specimens from Women with Low Plasma HIV-1 RNA Levels Typical of HIV-1 Nontransmitters |
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| المؤلفون: | Jared M. Baeten, Barbra A. Richardson, Julie Overbaugh, Kishorchandra Mandaliya, Sarah Benki, R. Scott McClelland, Sandra Emery, Ludo Lavreys |
| المصدر: | Journal of Clinical Microbiology. 44:4357-4362 |
| بيانات النشر: | American Society for Microbiology, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Adult, Microbiology (medical), Statistics as Topic, HIV Infections, Cervix Uteri, Polymerase Chain Reaction, Sensitivity and Specificity, Virus, law.invention, chemistry.chemical_compound, law, Virology, Humans, Polymerase chain reaction, Infectivity, biology, virus diseases, RNA, Provirus, biology.organism_classification, Body Fluids, Housekeeping gene, chemistry, DNA, Viral, Vagina, Lentivirus, HIV-1, RNA, Viral, Female, DNA |
| الوصف: | Studies of human immunodeficiency virus type 1 (HIV-1) transmission suggest that genital HIV-1 RNA and DNA may both be determinants of HIV-1 infectivity. Despite its potential role in HIV-1 transmission, there are limited quantitative data on genital HIV-1 DNA. Here we validated an in-house real-time PCR method for quantification of HIV-1 DNA in genital specimens. In reactions with 100 genomes to 1 genome isolated from a cell line containing one HIV-1 provirus/cell, this real-time PCR assay is linear and agrees closely with a commercially available real-time PCR assay specific for a cellular housekeeping gene. In mock genital samples spiked with low numbers of HIV-1-infected cells such that the expected HIV-1 DNA copy number/reaction was 100, 10, or 5, the average copy number/reaction was 80.2 (standard deviation [SD], 28.3), 9.1 (SD, 5.4), or 3.1 (SD, 2.1), respectively. We used this method to examine genital HIV-1 DNA levels in specimens from women whose low plasma HIV-1 RNA levels are typical of HIV-1 nontransmitters. The median HIV-1 DNA copy number in endocervical secretions from these women (1.8 HIV-1 DNA copies/10,000 cells) was lower than that for women with higher plasma HIV-1 RNA levels (16.6 HIV-1 DNA copies/10,000 cells) (P= 0.04), as was the median HIV-1 DNA copy number in vaginal secretions (undetectable versus 1.0 HIV-1 DNA copies/10,000 cells). These data suggest that women with low plasma HIV-1 RNA and thus a predicted low risk of HIV-1 transmission have low levels of genital HIV-1 cell-associated virus. The assay described here can be utilized in future efforts to examine the role of cell-associated HIV-1 in transmission. |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53e9a01cff658fafdea5741a627d579f https://doi.org/10.1128/jcm.01481-06 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....53e9a01cff658fafdea5741a627d579f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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