Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay
| العنوان: | Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay |
|---|---|
| المؤلفون: | Harinirina Aina Rabemananjara, Sandra Telfer, Jean Marc Reynes, Vololoniaina Raharinosy, Danielle Rakoto, Jean-Michel Heraud, Claudia Filippone |
| المساهمون: | Unité de Virologie [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Faculté des Sciences - Université d'Antananarivo, Université d'Antananarivo, Unité Peste - Plague Unit [Antananarivo, Madagascar], Institute of Biological and Environmental Sciences [Aberdeen], University of Aberdeen, Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections (UBIVE), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP), This study was supported by the Programme Transversal de Recherche (PTR 505) funded by the Institut Pasteur International Network. V.R. was also supported though Girard’s fellowship undergraduate program from the Institut Pasteur de Madagascar and traineeship grants Calmette and Yersin program from the Institut Pasteur International Network., We would like to express our gratitude to the staff of the Plague Central Laboratory Unit, Institut Pasteur de Madagascar: Minoarisoa Rajerison, Fehivola Mandanirina Andriamiarimanana, and Soanandrasana Rahelinirina for conducting the field work and for providing samples., Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Reynes, Jean-Marc, Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP) |
| المصدر: | Viruses Viruses, 2019, 11 (8), pp.718. ⟨10.3390/v11080718⟩ Viruses, MDPI, 2019, 11 (8), pp.718. ⟨10.3390/v11080718⟩ Viruses, Vol 11, Iss 8, p 718 (2019) |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Specific detection, 030106 microbiology, Thailand orthohantavirus, Genetic variants, lcsh:QR1-502, Computational biology, Biology, lcsh:Microbiology, 3. Good health, Real-time RT-PCR, Reverse transcription polymerase chain reaction, 03 medical and health sciences, Indian ocean, 030104 developmental biology, Infectious Diseases, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Virology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Hantavirus |
| الوصف: | International audience; Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53f276ff182762681dd4669ccbb2b2a5 https://hal-pasteur.archives-ouvertes.fr/pasteur-02545958/document |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....53f276ff182762681dd4669ccbb2b2a5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
|---|