Cell Origin Dictates Programming of Resident versus Recruited Macrophages during Acute Lung Injury
| العنوان: | Cell Origin Dictates Programming of Resident versus Recruited Macrophages during Acute Lung Injury |
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| المؤلفون: | Brian P. O'Connor, Stacey M. Thomas, Sonia M. Leach, William J. Janssen, Julie A. Reisz, Claudia Jakubzick, Donna L. Bratton, Alexandra L. McCubbrey, Angelo D'Alessandro, Thomas Danhorn, Michael P. Mohning, Kara J. Mould, Lea Barthel, Tasha E. Fingerlin |
| المصدر: | American Journal of Respiratory Cell and Molecular Biology. 57:294-306 |
| بيانات النشر: | American Thoracic Society, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pulmonary and Respiratory Medicine, Acute Lung Injury, Clinical Biochemistry, Cell, Inflammation, Biology, Lung injury, Proinflammatory cytokine, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Metabolomics, Macrophage, Cell Lineage, Glycolysis, Molecular Biology, Cell Proliferation, Original Research, Lung, Sequence Analysis, RNA, Gene Expression Profiling, Macrophages, Reproducibility of Results, RNA, Pneumonia, Cell Biology, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, 030228 respiratory system, Immunology, Cytokines, Female, Inflammation Mediators, medicine.symptom |
| الوصف: | Two populations of alveolar macrophages (AMs) coexist in the inflamed lung: resident AMs that arise during embryogenesis, and recruited AMs that originate postnatally from circulating monocytes. The objective of this study was to determine whether origin or environment dictates the transcriptional, metabolic, and functional programming of these two ontologically distinct populations over the time course of acute inflammation. RNA sequencing demonstrated marked transcriptional differences between resident and recruited AMs affecting three main areas: proliferation, inflammatory signaling, and metabolism. Functional assays and metabolomic studies confirmed these differences and demonstrated that resident AMs proliferate locally and are governed by increased tricarboxylic acid cycle and amino acid metabolism. Conversely, recruited AMs produce inflammatory cytokines in association with increased glycolytic and arginine metabolism. Collectively, the data show that even though they coexist in the same environment, inflammatory macrophage subsets have distinct immunometabolic programs and perform specialized functions during inflammation that are associated with their cellular origin. |
| تدمد: | 1535-4989 1044-1549 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::541fa0baaa4a47aeb335caef48b48cd1 https://doi.org/10.1165/rcmb.2017-0061oc |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....541fa0baaa4a47aeb335caef48b48cd1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15354989 10441549 |
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