Decitabine Improves the Clinical Manifestations of Rats Withl -NAME-Induced Pre-eclampsia: A Potential Approach to Studying Pre-eclampsia
| العنوان: | Decitabine Improves the Clinical Manifestations of Rats With |
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| المؤلفون: | Hui Du, Hao Liu, Ping Chen, Yuqi Li, Wanlu Liu, Yuanyuan Wu, Haiyi Liu, Xun Gong, Fuyuan Qiao, Xinwei Shi, Guiju Tang, Jing Cao |
| المصدر: | Hypertension in Pregnancy. 34:464-473 |
| بيانات النشر: | Informa UK Limited, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Eclampsia, business.industry, medicine.medical_treatment, Intraperitoneal injection, Maspin, Obstetrics and Gynecology, Decitabine, Pharmacology, Hypoxia (medical), medicine.disease, Blot, Blood pressure, medicine.anatomical_structure, Placenta, Immunology, Internal Medicine, Medicine, medicine.symptom, business, medicine.drug |
| الوصف: | Pre-eclampsia is a major cause of maternal mortality and morbidity. Conditions with low oxygen tension are regarded as a key factor. Decitabine can partly attenuate the effects of hypoxia. This research was designed to investigate the effects of decitabine in rats with NG-Nitro-L-arginine Methyl Eater (L-NAME) induced pre-eclampsia and to explore the molecular mechanisms.A Wistar rat model of pre-eclampsia was established by intraperitoneal injection of L-NAME, and the intervention reagent was decitabine. Blood pressure (BP) and 24-h urinary protein were monitored. The expression of Mammary Serine Protease Inhibitor (SERPINB5, maspin) in the placenta was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting.Systolic BP in the tail artery of pregnant rats was increased by more than 30 mm Hg, and 24-h urinary protein was significantly increased after L-NAME was added. After decitabine treatment, blood pressure and 24-h urinary protein were significantly decreased. The expression of SERPINB5 in the placenta significantly increased after L-NAME was added. Decitabine significantly elevated the expression of SERPINB5 in the placenta of rats with L-NAME-induced preeclampsia.Decitabine reduced 24-h urinary protein and partly decreased blood pressure of pre-eclampsia in late pregnancy in rats with L-NAME-induced pre-eclampsia and increased the expression of SERPINB5, but the molecular mechanism of decitabine's effect remains unknown. This research provided a potential approach to studying the pathogenesis, treatment and prevention of pre-eclampsia. |
| تدمد: | 1525-6065 1064-1955 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5420dcbc717f814bda1301ab45cb3d24 https://doi.org/10.3109/10641955.2015.1074245 |
| رقم الأكسشن: | edsair.doi.dedup.....5420dcbc717f814bda1301ab45cb3d24 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15256065 10641955 |
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