Highly active rubiscos discovered by systematic interrogation of natural sequence diversity
| العنوان: | Highly active rubiscos discovered by systematic interrogation of natural sequence diversity |
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| المؤلفون: | Niv Antonovsky, Ron Milo, Melina Shamshoum, Itai Sharon, Aia Oz, Yoav Peleg, Noam Prywes, David G. Wernick, Avi I. Flamholz, Benoit de Pins, Dina Hochhauser, Lior Shachar, Zhijun Guo, Dan Davidi, Shira Albeck, Oliver Mueller-Cajar, Yinon M. Bar-On, Jagoda Jabłońska |
| المصدر: | The EMBO Journal |
| مصطلحات موضوعية: | Resource, inorganic chemicals, Cyanobacteria, enhanced photosynthesis, Ribulose-Bisphosphate Carboxylase, carbon fixation, Plant Biology, carboxylation rate, General Biochemistry, Genetics and Molecular Biology, ribulose‐1,5‐bisphosphate carboxylase/oxygenase, 03 medical and health sciences, 0302 clinical medicine, Data Mining, metagenomic survey, Molecular Biology, 030304 developmental biology, 0303 health sciences, General Immunology and Microbiology, biology, General Neuroscience, fungi, RuBisCO, Carbon fixation, food and beverages, biology.organism_classification, Turnover number, Isoenzymes, Natural sequence, Metabolism, Biochemistry, biology.protein, Databases, Nucleic Acid, 030217 neurology & neurosurgery |
| الوصف: | CO 2 is converted into biomass almost solely by the enzyme rubisco. The poor carboxylation properties of plant rubiscos have led to efforts that made it the most kinetically characterized enzyme, yet these studies focused on 100) were active in vitro, with the fastest having a turnover number of 22 ± 1 s−1—sixfold faster than the median plant rubisco and nearly twofold faster than the fastest measured rubisco to date. Unlike rubiscos from plants and cyanobacteria, the fastest variants discovered here are homodimers and exhibit a much simpler folding and activation kinetics. Our pipeline can be utilized to explore the kinetic space of other enzymes of interest, allowing us to get a better view of the biosynthetic potential of the biosphere. Metagenomic and biochemical analysis of previously uncharacterized naturally‐occurring form‐II and II/III rubiscos leads to identification of an enzyme with the fastest CO2 fixation rate described to date. |
| اللغة: | English |
| تدمد: | 1460-2075 0261-4189 |
| DOI: | 10.15252/embj.2019104081 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::543525ca54f6df6eb86bc25ece8bed8f |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....543525ca54f6df6eb86bc25ece8bed8f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 02614189 |
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| DOI: | 10.15252/embj.2019104081 |