Cationic liposomes for generic signal amplification strategies in bioassays
| العنوان: | Cationic liposomes for generic signal amplification strategies in bioassays |
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| المؤلفون: | Axel Duerkop, Antje J. Baeumner, Carola Hofmann, Susanne Maerkl, Barbara Kaiser |
| المصدر: | Analytical and Bioanalytical Chemistry |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | ddc:540, Static Electricity, Sulforhodamine B, Biochemistry, Electrostatic interaction, Analytical Chemistry, law.invention, chemistry.chemical_compound, Dynamic light scattering, law, Cations, Escherichia coli, 570 Biowissenschaften, Biologie, Humans, Cationic liposome, Escherichia coli Infections, Phospholipids, Fluorescent Dyes, Chemiluminescence, Liposome, Liposomes . Electrostatic interaction . Bioanalysis . Bacteria . E. coli, Bacteria, Rhodamines, Chemistry, Vesicle, E. coli, Cationic polymerization, Combinatorial chemistry, Fluorescence, Bacterial Load, Spectrometry, Fluorescence, Bioanalysis, 540 Chemie, Liposomes, Luminescent Measurements, ddc:570, Research Paper |
| الوصف: | Liposomes have been widely applied in bioanalytical assays. Most liposomes used bare negative charges to prevent non-specific binding and increase colloidal stability. Here, in contrast, highly stable, positively charged liposomes entrapping the fluorescent dye sulforhodamine B (SRB) were developed to serve as a secondary, non-specific label‚ and signal amplification tool in bioanalytical systems by exploiting their electrostatic interaction with negatively charged vesicles, surfaces, and microorganisms. The cationic liposomes were optimized for long-term stability (> 5 months) and high dye entrapment yield. Their capability as secondary, non-specific labels was first successfully proven through electrostatic interactions of cationic and anionic liposomes using dynamic light scattering, and then in a bioassay with fluorescence detection leading to an enhancement factor of 8.5 without any additional surface blocking steps. Moreover, the cationic liposomes bound efficiently to anionic magnetic beads were stable throughout magnetic separation procedures and could hence serve directly as labels in magnetic separation and purification strategies. Finally, the electrostatic interaction was exploited for the direct, simple, non-specific labeling of gram-negative bacteria. Isolated Escherichia coli cells were chosen as models and direct detection was demonstrated via fluorescent and chemiluminescent liposomes. Thus, these cationic liposomes can be used as generic labels for the development of ultrasensitive bioassays based on electrostatic interaction without the need for additional expensive recognition units like antibodies, where desired specificity is already afforded through other strategies. Graphical abstract Electronic supplementary material The online version of this article (10.1007/s00216-020-02612-w) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| تدمد: | 1618-2650 1618-2642 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54c88ec7d7d03d43870e72b50870b356 https://doi.org/10.1007/s00216-020-02612-w |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....54c88ec7d7d03d43870e72b50870b356 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16182650 16182642 |
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