Seminal plasma miR-192a: a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair
| العنوان: | Seminal plasma miR-192a: a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair |
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| المؤلفون: | Peng Li, Zheng Li, Peng Xu, Guo-Qing Liang, Ruhui Tian, Erlei Zhi, Huixing Chen |
| المصدر: | Asian Journal of Andrology, Vol 20, Iss 4, Pp 396-399 (2018) Asian Journal of Andrology |
| بيانات النشر: | Medknow, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, Microsurgery, endocrine system, medicine.medical_specialty, Testicular tissue, Chinese men, Urology, Varicocele, 030232 urology & nephrology, Apoptosis, Semen, lcsh:RC870-923, miR-192a, nonobstructive azoospermia, seminal plasma, varicocelectomy, 03 medical and health sciences, 0302 clinical medicine, Asian People, Predictive Value of Tests, Testis, medicine, Humans, Nonobstructive azoospermia, Infertility, Male, Azoospermia, Cell Proliferation, 030219 obstetrics & reproductive medicine, Caspase 3, urogenital system, business.industry, General Medicine, lcsh:Diseases of the genitourinary system. Urology, medicine.disease, MicroRNAs, Treatment Outcome, Biomarker (medicine), Original Article, business, Biomarkers, Varicocele repair |
| الوصف: | This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA (miR)-192a levels in seminal plasma and testicular tissue were evaluated by quantitative real-time polymerase chain reaction from 60 men with nonobstructive azoospermia and varicoceles (Group A: 27 men with spermatozoa found in the ejaculate after surgery; Group B: 33 men without spermatozoa found in the ejaculate after surgery) and 30 controls. The seminal plasma and testicular tissue miR-192a levels were higher in Group B than in Group A and the controls (P < 0.001), and there was no significant difference between Group A and the controls (P > 0.05). Apoptosis and proliferation assays with miR mimics and inhibitors showed that miR-192a induced GC-2 cell apoptosis through the activation of Caspase-3 protein. Thus, seminal plasma miR-192a appears to be a potential marker for successfully indicating spermatozoa in the ejaculate following microsurgical varicocelectomy in men with nonobstructive azoospermia and varicoceles. Seminal plasma miR-192a may be a useful clinical marker for prescreening to determine which patients with nonobstructive azoospermia and varicoceles would benefit from varicocelectomy. |
| تدمد: | 1008-682X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5502fc500d6200df27354db0390ba798 https://doi.org/10.4103/aja.aja_8_18 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5502fc500d6200df27354db0390ba798 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1008682X |
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