Genome size and gas chromatography-mass spectrometry (GC–MS) analysis of field-grown and in vitro regenerated Pluchea lanceolata plants
| العنوان: | Genome size and gas chromatography-mass spectrometry (GC–MS) analysis of field-grown and in vitro regenerated Pluchea lanceolata plants |
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| المؤلفون: | Jyoti, Mamgain, A, Mujib, Rukaya, Syeed, Bushra, Ejaz, Moien Qadir, Malik, Yashika, Bansal |
| المصدر: | Journal of Applied Genetics. 64:1-21 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Genetics, General Medicine |
| الوصف: | Pluchea lanceolata is a threatened pharmacologically important plant from the family Asteraceae. It is a source of immunologically active compounds; large-scale propagation may offer compounds with medicinal benefits. Traditional propagation method is ineffective as the seeds are not viable; and root sprout propagation is a slow process and produces less numbers of plants. Plant tissue culture technique is an alternative, efficient method for increasing mass propagation and it also facilitate genetic improvement. The present study investigated a three-way regeneration system in P. lanceolata using indirect shoot regeneration (ISR), direct shoot regeneration (DSR), and somatic embryo mediated regeneration (SER). Aseptic leaf and nodal explants were inoculated on Murashige and Skoog (MS) medium amended with plant growth regulators (PGRs), 2,4-dichlorophenoxy acetic acid (2,4-D), 1-naphthalene acetic acid (NAA), and 6-benzyl amino purine (BAP) either singly or in combinations. Compact, yellowish green callus was obtained from leaf explants in 1.0 mg/l BAP (89.10%) added medium; ISR percentage was high, i.e., 69.33% in 2.0 mg/l BAP + 0.5 mg/l NAA enriched MS with 4.02 mean number of shoots per callus mass. Highest DSR frequency (67.15%) with an average of 5.62 shoot numbers per explant was noted in 0.5 mg/l BAP added MS medium. Somatic embryos were produced in 1.0 mg/l NAA fortified medium with 4.1 mean numbers of somatic embryos per culture. On BAP (1.0 mg/l) + 0.5 mg/l gibberellic acid (GA |
| تدمد: | 2190-3883 1234-1983 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::559416aaa2f985cdcc13c3948610783b https://doi.org/10.1007/s13353-022-00727-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....559416aaa2f985cdcc13c3948610783b |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 21903883 12341983 |
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