Bone marrow-derived mesenchymal stem cells ameliorate severe acute pancreatitis by inhibiting necroptosis in rats
| العنوان: | Bone marrow-derived mesenchymal stem cells ameliorate severe acute pancreatitis by inhibiting necroptosis in rats |
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| المؤلفون: | Hongbo Meng, Bo Zhou, Guodong Song, Zhenshun Song, Jia Zhou, Dalu Liu, Zhilong Ma, Daohai Qian |
| المصدر: | Molecular and Cellular Biochemistry. 459:7-19 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Indoles, Necrosis, Necroptosis, Clinical Biochemistry, Bone Marrow Cells, Protein Serine-Threonine Kinases, Mesenchymal Stem Cell Transplantation, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, medicine, Animals, Protein kinase A, Molecular Biology, Cell Death, Tumor Necrosis Factor-alpha, business.industry, Mesenchymal stem cell, Imidazoles, Mesenchymal Stem Cells, Cell Biology, General Medicine, Allografts, medicine.disease, Rats, 030104 developmental biology, medicine.anatomical_structure, Pancreatitis, Receptor-Interacting Protein Serine-Threonine Kinases, 030220 oncology & carcinogenesis, Acute Disease, Cancer research, Acute pancreatitis, Bone marrow, medicine.symptom, Pancreatic injury, business, Protein Kinases |
| الوصف: | The treatment and prognosis for severe acute pancreatitis (SAP) is currently unsatisfactory showing a high incidence of morbidity and mortality. Here, we investigated the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on SAP in rats and explored the possible mechanisms. The common bile duct of each model rat was occluded at the liver hilum, and the induction of SAP was achieved by retrograde perfusion of 3% sodium taurocholate (NaT). Prepared BMSCs were intravenously injected via the tail vein. Pancreatic acinar cells (PACs) were isolated from rat pancreas, and induced by TNF-α. In the present study, we found that necroptosis was activated in NaT-induced acute-necrotized pancreatitis, and transplanted BMSCs could inhibit necroptosis, repair pancreatic injury, and reduce systemic inflammatory response. In addition, necrostatin-1 (Nec-1), as the inhibitor of receptor-interacting protein kinase 1 (RIPK1), could also reduce SAP to some extent. Besides, we detected that BMSCs could also promote regeneration of damaged pancreatic tissues. Furthermore, in vitro, we also investigated that BMSCs could suppress TNF-α-induced necroptosis and improve the viability of PACs. In addition, Nec-1 and knockdown of receptor-interacting protein kinase 3 (RIPK3) or mixed lineage kinase domain-like protein (MLKL) could also inhibit necrosis of PACs induced by TNF-α. BMSCs ameliorated SAP and reduced injury of PACs by suppressing the activation of the necroptosis signaling pathway. |
| تدمد: | 1573-4919 0300-8177 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::559e9dd0a030bf4ffdfc4da6184d4193 https://doi.org/10.1007/s11010-019-03546-3 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....559e9dd0a030bf4ffdfc4da6184d4193 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15734919 03008177 |
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