A human breast atlas integrating single-cell proteomics and transcriptomics
| العنوان: | A human breast atlas integrating single-cell proteomics and transcriptomics |
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| المؤلفون: | G. Kenneth Gray, Carman Man-Chung Li, Jennifer M. Rosenbluth, Laura M. Selfors, Nomeda Girnius, Jia-Ren Lin, Ron C.J. Schackmann, Walter L. Goh, Kaitlin Moore, Hana K. Shapiro, Shaolin Mei, Kurt D’Andrea, Katherine L. Nathanson, Peter K. Sorger, Sandro Santagata, Aviv Regev, Judy E. Garber, Deborah A. Dillon, Joan S. Brugge |
| المصدر: | Developmental cell, vol 57, iss 11 Dev Cell |
| بيانات النشر: | Elsevier BV, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Proteomics, Aging, 1.1 Normal biological development and functioning, Breast Neoplasms, multiplexed tissue staining, multi-omic single-cell atlas, Medical and Health Sciences, Article, General Biochemistry, Genetics and Molecular Biology, Mammary Glands, Animal, breast cancer, Pregnancy, Underpinning research, Genetics, Animals, Humans, 2.1 Biological and endogenous factors, Breast, Aetiology, Molecular Biology, organoids, Cancer, mammary biology, Animal, Human Genome, Cell Biology, Biological Sciences, BRCA1, CyTOF mass cytometry, Mammary Glands, BRCA2, cell state plasticity, scRNA-Seq, Female, Transcriptome, Biotechnology, Developmental Biology |
| الوصف: | The breast is a dynamic organ whose response to physiological and pathophysiological conditions alters its disease susceptibility, yet the specific effects of these clinical variables on cell state remain poorly annotated. We present a unified, high-resolution breast atlas by integrating single-cell RNA-seq, mass cytometry, and cyclic immunofluorescence, encompassing a myriad of states. We define cell subtypes within the alveolar, hormone-sensing, and basal epithelial lineages, delineating associations of several subtypes with cancer risk factors, including age, parity, and BRCA2 germline mutation. Of particular interest is a subset of alveolar cells, termed basal-luminal (BL) cells, which exhibits poor transcriptional lineage fidelity, accumulates with age, and carries a gene signature associated with basal-like breast cancer. We further utilize a medium-depletion approach to identify molecular factors regulating cell subtype proportion in organoids. Together, these data are a rich resource to elucidate diverse mammary cell states. |
| تدمد: | 1534-5807 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55a7ff61937298814166a3ca021354eb https://doi.org/10.1016/j.devcel.2022.05.003 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....55a7ff61937298814166a3ca021354eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15345807 |
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