Bushen Huoxue recipe attenuates early pregnancy loss via activating endometrial COX2-PGE2 angiogenic signaling in mice
| العنوان: | Bushen Huoxue recipe attenuates early pregnancy loss via activating endometrial COX2-PGE2 angiogenic signaling in mice |
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| المؤلفون: | Mingmin Zhang, Chu Zhang, Fanru Zhou, Yufan Song, Fan Li, Xia Liu, Xiujuan Tan, Wenxin Zhu, Runan Hu, Jiahui Ding, Wenwen Ma |
| المصدر: | BMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-15 (2021) BMC Complementary Medicine and Therapies |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Endometrial angiogenesis, Early Pregnancy Loss, medicine.medical_treatment, Early pregnancy loss, Neovascularization, Physiologic, Controlled ovarian hyperstimulation, Endometrium, Dinoprostone, Human chorionic gonadotropin, Andrology, 03 medical and health sciences, Mice, 0302 clinical medicine, Traditional Chinese medicine, Pregnancy, Medicine, Animals, Embryo Implantation, Medicine, Chinese Traditional, 030219 obstetrics & reproductive medicine, In vitro fertilisation, business.industry, Hormone balance, lcsh:Other systems of medicine, medicine.disease, lcsh:RZ201-999, Embryo transfer, Abortion, Spontaneous, Pregnancy rate, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Complementary and alternative medicine, Cyclooxygenase 2, Female, Bushen Huoxue recipe, business, Research Article, Drugs, Chinese Herbal, Signal Transduction |
| الوصف: | Background During the fresh cycles of in vitro fertilization and embryo transfer, a disturbance in the reproductive endocrine environment following controlled ovarian hyperstimulation (COH) is closely related to compromised endometrial receptivity. This is a major disadvantage for women during pregnancy. Based on the theory of traditional Chinese medicine, Bushen Huoxue recipe (BSHXR) has been indicated to facilitate embryo implantation. Methods The COH model (Kunming breed) was induced by injecting mice with pregnant mare serum gonadotrophin (0.4 IU/g) and human chorionic gonadotropin (1 IU/g), followed by treatment with BSHXR at three different concentrations (5.7, 11.4, and 22.8 g/kg), Bushen recipe (BSR) (5.7 g/kg), and Huoxue recipe (HXR) (5.7 g/kg). After successful mating, the pregnancy rate and implantation sites were examined on embryo day 8 (ED8), and the weight ratio of endometrium was calculated on ED4 midnight. Serum estrogen, progesterone, and endometrial PGE2 levels were measured using enzyme-linked immunosorbent assay. The endometrial microvasculature was evaluated using CD31 immunostaining. The protein and mRNA levels of the angiogenic factors in the endometrium were evaluated using western blot, immunohistochemistry, and polymerase chain reaction. Results In the COH group, the pregnancy rate and implantation sites were significantly decreased, and abnormal serum hormone levels and impaired endometrial vascular development were observed. After BSHXR treatment, the supraphysiological serum progesterone level in COH mice was restored to normalcy. Moreover, the abnormal expression of the endometrial pro-angiogenic factors, including HIF1α, COX2-PGE2 pathway, and the down-stream factors, namely, MMP2, MMP9, TIMP2, and FGF2 after subjecting mice to COH was significantly improved after BSHXR treatment. Conclusion BSHXR could improve embryo implantation by regulating hormonal balance and modulating endometrial angiogenesis in mice, without inducing any side effects in normal pregnancy. |
| اللغة: | English |
| تدمد: | 2662-7671 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55bb06732b48ab5c9f29f702a03529bc https://doaj.org/article/dc00f76c1d0642f2bd8c3ebeadd6de33 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....55bb06732b48ab5c9f29f702a03529bc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26627671 |
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