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Bio‐assembling Macro‐Scale, Lumenized Airway Tubes of Defined Shape via Multi‐Organoid Patterning and Fusion

التفاصيل البيبلوغرافية
العنوان: Bio‐assembling Macro‐Scale, Lumenized Airway Tubes of Defined Shape via Multi‐Organoid Patterning and Fusion
المؤلفون: Yan Yan Shery Huang, Eugene M. Terentjev, Fanlong Meng, Antranik Mavousian, Bernhard Strauss, Karim Ouaras, Corrado Mazzaglia, Joo-Hyeon Lee, Callum Macintosh, Catherine Dabrowska, Ye Liu
المساهمون: Lee, Joo‐Hyeon [0000-0002-7364-6422], Huang, Yan Yan Shery [0000-0003-2619-730X], Apollo - University of Cambridge Repository, Department of Engineering [Cambridge], University of Cambridge [UK] (CAM)
المصدر: Advanced Science
Advanced Science, Wiley Open Access, 2021, 8 (9), pp.2003332. ⟨10.1002/advs.202003332⟩
Advanced Science, Vol 8, Iss 9, Pp n/a-n/a (2021)
بيانات النشر: Apollo - University of Cambridge Repository, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Scaffold, Materials science, Science, General Chemical Engineering, General Physics and Astronomy, Medicine (miscellaneous), 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Genetics and Molecular Biology (miscellaneous), PHYSICAL FORCES, [SPI.MAT]Engineering Sciences [physics]/Materials, Cell Fusion, Tissue Culture Techniques, Mice, Tissue engineering, Biomimetics, Morphogenesis, Organoid, Animals, General Materials Science, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Process (anatomy), organoids, Fusion, Full Paper, Stem Cells, [SPI.PLASMA]Engineering Sciences [physics]/Plasmas, General Engineering, bio‐assembly, Full Papers, 021001 nanoscience & nanotechnology, respiratory system, 0104 chemical sciences, Trachea, Macroscopic scale, tissue engineering, Models, Animal, 0210 nano-technology, tissue morphogenesis, Lumen (unit), Biomedical engineering
الوصف: Epithelial, stem‐cell derived organoids are ideal building blocks for tissue engineering, however, scalable and shape‐controlled bio‐assembly of epithelial organoids into larger and anatomical structures is yet to be achieved. Here, a robust organoid engineering approach, Multi‐Organoid Patterning and Fusion (MOrPF), is presented to assemble individual airway organoids of different sizes into upscaled, scaffold‐free airway tubes with predefined shapes. Multi‐Organoid Aggregates (MOAs) undergo accelerated fusion in a matrix‐depleted, free‐floating environment, possess a continuous lumen, and maintain prescribed shapes without an exogenous scaffold interface. MOAs in the floating culture exhibit a well‐defined three‐stage process of inter‐organoid surface integration, luminal material clearance, and lumina connection. The observed shape stability of patterned MOAs is confirmed by theoretical modelling based on organoid morphology and the physical forces involved in organoid fusion. Immunofluorescent characterization shows that fused MOA tubes possess an unstratified epithelium consisting mainly of tracheal basal stem cells. By generating large, shape‐controllable organ tubes, MOrPF enables upscaled organoid engineering towards integrated organoid devices and structurally complex organ tubes.
A robust organoid upscaling and engineering approach, Multi‐Organoid Patterning and Fusion (MOrPF), is presented to assemble individual airway organoids of different sizes into scaffold‐free airway tubes of anatomically relevant size and shape. Airway organoid fusion undergoes inter‐organoid surface integration, luminal material clearance, and lumina connection. MOrPF enables shape‐defined organoid engineering toward integrated organoid devices and structurally complex organ tubes.
وصف الملف: text/xml; application/pdf
تدمد: 2198-3844
DOI: 10.17863/cam.69413
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55c3a39f3394d3a0d9f058831502ef91
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....55c3a39f3394d3a0d9f058831502ef91
قاعدة البيانات: OpenAIRE
الوصف
تدمد:21983844
DOI:10.17863/cam.69413