Programming Structured DNA Assemblies to Probe Biophysical Processes
| العنوان: | Programming Structured DNA Assemblies to Probe Biophysical Processes |
|---|---|
| المؤلفون: | Hyungmin Jun, Tyson R. Shepherd, Remi Veneziano, Mark Bathe, William P. Bricker, James L. Banal, Molly F Parsons, Eike-Christian Wamhoff, Matthew B. Stone, Xiao Wang |
| المساهمون: | Massachusetts Institute of Technology. Department of Biology, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Mechanical Engineering |
| المصدر: | PMC |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Interface (Java), Biophysics, Bioengineering, Nanotechnology, 02 engineering and technology, Biochemistry, Article, 03 medical and health sciences, chemistry.chemical_compound, Structural Biology, Biomimetics, DNA nanotechnology, DNA origami, Receptors, Immunologic, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Biomolecule, Cell Biology, DNA, 021001 nanoscience & nanotechnology, Folding (chemistry), Structural biology, chemistry, Biophysical Process, RNA, 0210 nano-technology, Signal Transduction |
| الوصف: | Structural DNA nanotechnology is beginning to emerge as a widely accessible research tool to mechanistically study diverse biophysical processes. Enabled by scaffolded DNA origami in which a long single strand of DNA is weaved throughout an entire target nucleic acid assembly to ensure its proper folding, assemblies of nearly any geometric shape can now be programmed in a fully automatic manner to interface with biology on the 1-100-nm scale. Here, we review the major design and synthesis principles that have enabled the fabrication of a specific subclass of scaffolded DNA origami objects called wireframe assemblies. These objects offer unprecedented control over the nanoscale organization of biomolecules, including biomolecular copy numbers, presentation on convex or concave geometries, and internal versus external functionalization, in addition to stability in physiological buffer. To highlight the power and versatility of this synthetic structural biology approach to probing molecular and cellular biophysics, we feature its application to three leading areas of investigation: light harvesting and nanoscale energy transport, RNA structural biology, and immune receptor signaling, with an outlook toward unique mechanistic insight that may be gained in these areas in the coming decade. United States. Office of Naval Research (Grant N00014-12-1-0621) United States. Office of Naval Research (Grant N00014-14-1-0609) United States. Office of Naval Research (Grant N00014-13-1-0664) United States. Office of Naval Research (Grant N00014-16-1-2506) United States. Office of Naval Research (Grant N00014-16-1-2181) United States. Office of Naval Research (Grant N00014-16-1-2953) United States. Office of Naval Research (Grant N00014-17-1-2609) United States. Office of Naval Research (Grant N00014-18-1-2290) National Science Foundation (U.S.) (Grant CCF-1547999) National Science Foundation (U.S.) (Grant CCF-1564025) National Science Foundation (U.S.) (Grant CMMI-1334109) National Science Foundation (U.S.) (Grant CBET-1729397) National Science Foundation (U.S.) (Grant CHE-1839155) National Science Foundation (U.S.) (Grant PHY-1305537) National Science Foundation (U.S.) (Grant PHY-1707999) United States. Army Research Office (Grant W911NF1210420) Human Frontier Science Program (Strasbourg, France) (Grant RGP0029/2014) United States. Department of Energy (Grant DE-SC0016353) United States. Department of Energy (Grant DE-SC0001088 National Institutes of Health (U.S.) (Grant U01-MH106011) National Institutes of Health (U.S.) (Grant R01-MH112694) National Institutes of Health (U.S.) (Grant R21-EB026008) National Institute of Environmental Health Sciences (Grant (P30-ES002109) |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55d37f7fb4bcd94578258326e7698d33 https://europepmc.org/articles/PMC7035826/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....55d37f7fb4bcd94578258326e7698d33 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |