The 5'-untranslated regions of picornavirus RNAs contain independent functional domains essential for RNA replication and translation
| العنوان: | The 5'-untranslated regions of picornavirus RNAs contain independent functional domains essential for RNA replication and translation |
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| المؤلفون: | Wendy S. Barclay, David J.A. Evans, J B Rohll, R Ley, N Percy, Jeffrey W. Almond |
| المصدر: | University of St Andrews CRIS |
| بيانات النشر: | American Society for Microbiology, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Chloramphenicol O-Acetyltransferase, Untranslated region, Picornavirus, viruses, Molecular Sequence Data, Immunology, Picornaviridae, Virus Replication, Microbiology, Chloramphenicol acetyltransferase, Virology, Humans, Replicon, Genomic organization, Recombination, Genetic, Genetics, Base Sequence, biology, RNA, biology.organism_classification, Introns, Internal ribosome entry site, Viral replication, Protein Biosynthesis, Insect Science, Nucleic Acid Conformation, RNA, Viral, Research Article, HeLa Cells |
| الوصف: | The role of the 5'-untranslated region (5'UTR) in the replication of enteroviruses has been studied by using a series of poliovirus type 3 (PV3) replicons containing the chloramphenicol acetyltransferase reporter gene in which the 5'UTR was replaced by the 5'UTR of either coxsackievirus B4 or human rhinovirus 14 or composite 5'UTRs derived from sequences of PV3, human rhinovirus 14, coxsackievirus B4, or encephalomyocarditis virus. The results indicate that efficient replication of an enterovirus genome requires a compatible interaction between the 5'-terminal cloverleaf structure and the coding and/or 3'-noncoding regions of the genome. A crucial determinant of this interaction is the stem-loop formed by nucleotides 46 to 81 (stem-loop d). The independence of the cloverleaf structure formed by the 5'-terminal 88 nucleotides and the ribosome landing pad or internal ribosome entry site (IRES) was investigated by constructing a 5'UTR composed of the PV3 cloverleaf and the IRES from encephalomyocarditis virus. Chloramphenicol acetyltransferase gene-containing replicons and viruses containing this recombinant 5'UTR showed levels of replication similar to those of the corresponding genomes containing the complete PV3 5'UTR, indicating that the cloverleaf and the IRES may be regarded as functionally independent and nonoverlapping elements. |
| تدمد: | 1098-5514 0022-538X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55d519c72f1ccb458e0bdb497740da66 https://doi.org/10.1128/jvi.68.7.4384-4391.1994 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....55d519c72f1ccb458e0bdb497740da66 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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