Establishment of a monoclonal antibody against glycosylated CD271 specific for cancer cells in immunohistochemistry
| العنوان: | Establishment of a monoclonal antibody against glycosylated CD271 specific for cancer cells in immunohistochemistry |
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| المؤلفون: | Keitaro Fujii, Shinkichi Morita, Mai Mochizuki, Rie Shibuya‐Takahashi, Haruna Fujimori, Kazunori Yamaguchi, Jiro Abe, Tomoko Yamazaki, Takayuki Imai, Kazuo Sugamura, Jun Yasuda, Kennichi Satoh, Ikuro Sato, Ryoko Saito‐Koyama, Fumiyoshi Fujishima, Hironobu Sasano, Yukinari Kato, Kazuto Matsuura, Yukinori Asada, Keiichi Tamai |
| المصدر: | Cancer Science. 113:2878-2887 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Barrett Esophagus, Mice, Cancer Research, Glycosylation, Esophageal Neoplasms, Oncology, Carcinoma, Squamous Cell, Animals, Antibodies, Monoclonal, Receptors, Nerve Growth Factor, General Medicine, Adapalene, Immunohistochemistry |
| الوصف: | Various proteins are highly expressed in cancer (e.g., epidermal growth factor receptor); however, the majority are also expressed in normal cells, although they may differ in expression intensity. Recently, we reported that CD271 (nerve growth factor receptor), a glycosylated protein, increases malignant behavior of cancer, particularly stemlike phenotypes in squamous cell carcinoma (SCC). CD271 is expressed in SCC and in normal epithelial basal cells. Glycosylation alterations generally occur in cancer cells; therefore, we attempted to establish a cancer-specific anti-glycosylated CD271 antibody. We purified recombinant glycosylated CD271 protein, immunized mice with the protein, and screened hybridomas using an ELISA assay with cancer cell lines. We established a clone G4B1 against CD271 which is glycosylated with O-glycan and sialic acid. The G4B1 antibody reacted with the CD271 protein expressed in esophageal cancer, but not in normal esophageal basal cells. This specificity was confirmed in hypopharyngeal and cervical cancers. G4B1 antibody recognized the fetal esophageal epithelium and Barrett's esophagus, which possess stem cell-like characteristics. In conclusion, G4B1 antibody could be useful for precise identification of dysplasia and cancer cells in SCC. |
| تدمد: | 1349-7006 1347-9032 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55db741bbdf4920db2993b9064b24528 https://doi.org/10.1111/cas.15340 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....55db741bbdf4920db2993b9064b24528 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13497006 13479032 |
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