Molecular epidemiology and mechanisms of tigecycline resistance in carbapenem‐resistant Klebsiella pneumoniae isolates
| العنوان: | Molecular epidemiology and mechanisms of tigecycline resistance in carbapenem‐resistant Klebsiella pneumoniae isolates |
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| المؤلفون: | Qute Choi, Sun Hoe Koo, Gye Cheol Kwon, Yumi Park |
| المصدر: | Journal of Clinical Laboratory Analysis |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), tigecycline resistance, Klebsiella pneumoniae, Clinical Biochemistry, Microbial Sensitivity Tests, Tigecycline, Biology, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Drug Resistance, Bacterial, Pulsed-field gel electrophoresis, medicine, Humans, Immunology and Allergy, Gene, Research Articles, Molecular Epidemiology, Molecular epidemiology, Biochemistry (medical), Public Health, Environmental and Occupational Health, Hematology, biology.organism_classification, Anti-Bacterial Agents, Klebsiella Infections, SOXS, Medical Laboratory Technology, Carbapenem-Resistant Enterobacteriaceae, 030104 developmental biology, 030220 oncology & carcinogenesis, Mutation, Multilocus sequence typing, Efflux, carbapenem‐resistant Klebsiella pneumoniae, Carrier Proteins, Research Article, AcrAB‐TolC efflux pump, medicine.drug |
| الوصف: | Background The emergence and transmission of tigecycline‐ and carbapenem‐resistant Klebsiella pneumoniae (TCRKP) have become a major concern to public health globally. Here, we investigated the molecular epidemiology and mechanisms of tigecycline resistance in carbapenem‐resistant K pneumoniae (CRKP) isolates. Methods Forty‐five non‐duplicate CRKP isolates were collected from January 2017 to June 2019. We performed antimicrobial susceptibility tests, multilocus sequence typing (MLST), and pulsed‐field gel electrophoresis (PFGE). PCR and DNA sequencing were performed for the detection and mutation analysis of acrR, oqxR, ramR, rpsJ, tet(A), and tet(X) genes, which are related to tigecycline resistance. The expression levels of efflux pump genes acrB and oqxB and their regulator genes rarA, ramA, soxS, and marA were assessed by quantitative real‐time PCR. Results The resistance rate to tigecycline in CRKP isolates was 37.8% (17/45). K pneumoniae ST307 was a predominant clone type (70.6%, 12/17) among the TCRKP isolates. The expression levels of acrB (P We introduce the mechanisms of tigecycline resistance in carbapenem‐resistant K pneumoniae (CRKP) isolates. The transcriptional activator MarA‐mediated overexpression of AcrAB efflux pump plays an important role in the emergence of tigecycline resistance in CRKP isolates. |
| تدمد: | 1098-2825 0887-8013 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56c4e497a5dec058f63f021ae2fab30b https://doi.org/10.1002/jcla.23506 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....56c4e497a5dec058f63f021ae2fab30b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982825 08878013 |
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