Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator
| العنوان: | Enhanced Luminescent Detection of Circulating Tumor Cells by a 3D Printed Immunomagnetic Concentrator |
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| المؤلفون: | Kisung Ko, Abdurhaman Teyib Abafogi, Dinesh Veeran Ponnuvelu, Sungsu Park, Ilchan Song, Chanyong Park |
| المصدر: | Biosensors, Vol 11, Iss 278, p 278 (2021) Biosensors Volume 11 Issue 8 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 3d printed, Luminescence, Clinical Biochemistry, Cell Count, circulating tumor cells, Immunomagnetic separation, ATP luminescence assay, Article, Carboplatin, Circulating tumor cell, Antineoplastic Combined Chemotherapy Protocols, Humans, Cyclophosphamide, Detection limit, Chemistry, General Medicine, 3D printing, Neoplastic Cells, Circulating, immunomagnetic separation, Molecular biology, Luminescent Measurements, Printing, Three-Dimensional, Cancer cell, High flow, Thiotepa, TP248.13-248.65, Biotechnology |
| الوصف: | Circulating tumor cells (CTCs) are an indicator of metastatic progression and relapse. Since non-CTC cells such as red blood cells outnumber CTCs in the blood, the separation and enrichment of CTCs is key to improving their detection sensitivity. The ATP luminescence assay can measure intracellular ATP to detect cells quickly but has not yet been used for CTC detection in the blood because extracellular ATP in the blood, derived from non-CTCs, interferes with the measurement. Herein, we report on the improvement of the ATP luminescence assay for the detection of CTCs by separating and concentrating CTCs in the blood using a 3D printed immunomagnetic concentrator (3DPIC). Because of its high-aspect-ratio structure and resistance to high flow rates, 3DPIC allows cancer cells in 10 mL to be concentrated 100 times within minutes. This enables the ATP luminescence assay to detect as low as 10 cells in blood, thereby being about 10 times more sensitive than when commercial kits are used for CTC concentration. This is the first time that the ATP luminescence assay was used for the detection of cancer cells in blood. These results demonstrate the feasibility of 3DPIC as a concentrator to improve the detection limit of the ATP luminescence assay for the detection of CTCs. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2079-6374 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56cbc956a08eee65e1d3df839b8b9e7a https://www.mdpi.com/2079-6374/11/8/278 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....56cbc956a08eee65e1d3df839b8b9e7a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20796374 |
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