Simultaneous determination and pharmacokinetic study of six components in beagle dog plasma by UPLC–MS/MS after oral administration of Astragalus Membranaceus aqueous extract
| العنوان: | Simultaneous determination and pharmacokinetic study of six components in beagle dog plasma by UPLC–MS/MS after oral administration of Astragalus Membranaceus aqueous extract |
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| المؤلفون: | Xin Yu, Kexin Xia, Siyang Wu, Qiutao Wang, Wenhao Cheng, Chun Ji, Wei Yang, Chen Kang, Zheng Yuan, Yingfei Li |
| المصدر: | Biomedical Chromatography. 36 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Pharmacology, Clinical Biochemistry, Administration, Oral, Water, Reproducibility of Results, General Medicine, Astragalus propinquus, Biochemistry, Analytical Chemistry, Dogs, Tandem Mass Spectrometry, Drug Discovery, Animals, Molecular Biology, Chromatography, High Pressure Liquid, Chromatography, Liquid |
| الوصف: | Astragalus Membranaceus (AM) is widely applied in Chinese herbal compound formulas for treating various kinds of diseases. However, relative pharmacokinetics data on AM in nonrodents is still lacking. Here, an UPLC-MS/MS method for determining the six main compounds of AM was developed. The chromatographic separation was carried out by a Waters Acquity UPLC HSS T3 column (100 × 2.1 mm, 1.8 μm) with gradient elution of water-formic acid (99.98:0.02, v/v) and acetonitrile-formic acid (99.98:0.02, v/v) at a flow rate of 0.3 ml/min within 11 min. Analyses of all compounds were conducted in multiple reaction monitoring mode with a positive/negative ion-switching mode of an electrospray ionization source in a single run. The analytical method was validated in terms of specificity, linearity, accuracy, precision, stability, etc. The method showed excellent linearity (r 0.999) over certain concentration ranges. The intra-day and inter-day precisions were evaluated, and the RSD values were12.4%. Furthermore, the validated method was successfully applied to determine the six components in plasma after oral administration of AM aqueous extract to beagle dogs and the pharmacokinetic parameters were obtained. Together, this study provides a reference for medication in the clinical practice of AM. |
| تدمد: | 1099-0801 0269-3879 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::576ce28e765019549fb875f3fc42fa26 https://doi.org/10.1002/bmc.5488 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....576ce28e765019549fb875f3fc42fa26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990801 02693879 |
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