In vitro expression and analysis of the 826 human G protein-coupled receptors
| العنوان: | In vitro expression and analysis of the 826 human G protein-coupled receptors |
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| المؤلفون: | Lu Zhang, John J. Skinner, Na Chen, Vsevolod Katritch, Dong Wu, Angela L. Walker, Junlin Liu, Qiaoyun Shi, Raymond C. Stevens, Lu Xue, Zeshi Wang, Pei Si, Xuechen Lv, Lixia Zhao, Zhi-Jie Liu, Xiangxiang Gu, Qiwen Tan |
| المصدر: | Protein & Cell Protein & Cell, Vol 7, Iss 5, Pp 325-337 (2016) |
| بيانات النشر: | Oxford University Press (OUP), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Animal biochemistry, Gene Expression, Computational biology, Spodoptera, Biology, Crystallography, X-Ray, Receptors, Odorant, Biochemistry, Receptors, G-Protein-Coupled, 03 medical and health sciences, G protein-coupled receptors, Protein Domains, Drug Discovery, Sf9 Cells, Extracellular, Animals, Humans, lcsh:QH573-671, Receptor, lcsh:QP501-801, protein expression, surface expression analysis, G protein-coupled receptor, lcsh:Cytology, Receptors, Purinergic P1, Computational Biology, Cell Biology, In vitro, Human genetics, 030104 developmental biology, Expression (architecture), fusion construct, insect, Receptors, Adrenergic, beta-1, Stem cell, Developmental biology, Research Article, Plasmids, Biotechnology |
| الوصف: | G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826 human GPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility. Electronic supplementary material The online version of this article (doi:10.1007/s13238-016-0263-8) contains supplementary material, which is available to authorized users. |
| تدمد: | 1674-8018 1674-800X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5775d776ddd73c80886542088a4bc70c https://doi.org/10.1007/s13238-016-0263-8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5775d776ddd73c80886542088a4bc70c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16748018 1674800X |
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