A six‐colour flow cytometric method for simultaneous detection of cell phenotype and apoptosis of circulating endothelial cells
| العنوان: | A six‐colour flow cytometric method for simultaneous detection of cell phenotype and apoptosis of circulating endothelial cells |
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| المؤلفون: | S. Mariucci, B. Rovati, M. Danova, Sara Delfanti, S. Chatzileontiadou, K. Bencardino, M. Manzoni |
| المصدر: | Scandinavian Journal of Clinical and Laboratory Investigation. 69:433-438 |
| بيانات النشر: | Informa UK Limited, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Adult, Male, medicine.drug_class, Clinical Biochemistry, Color, Apoptosis, Biology, Monoclonal antibody, Sensitivity and Specificity, Flow cytometry, Immunophenotyping, Annexin, medicine, Humans, Annexin A5, Progenitor cell, Blood Cells, medicine.diagnostic_test, Endothelial Cells, General Medicine, Middle Aged, Flow Cytometry, Phenotype, Staining, Cell biology, Dactinomycin, Female |
| الوصف: | Blood circulating endothelial cells (CECs), with their resting and activated subsets, (rCECs and aCECs) and circulating progenitors cells (CEPs) are two extremely rare cell populations that are important in tissue vascularization. Their number and function are modulated in diseases involving vascular injury, such as human tumours. Although a consensus on the phenotypic definition of endothelial cells, as well as on the optimal enumeration technique, is still lacking, the number of clinical studies based on assessment of these cells is rapidly expanding, as well as the analytical methods employed. The present study aimed to develop a rapid and sensitive flow cytometric method of quantifying and characterizing CECs (with both their subsets and the apoptotic fraction) and CEPs. We analysed peripheral blood samples from 21 subjects with a six-colour flow cytometric approach allowing detection of the cell phenotype of CECs and CEPs using a monoclonal antibodies panel and a dedicated gating strategy. Apoptotic CECs were detected with Annexin V and dead cells with 7-amino-actinomycin D staining. The described technique proved to be a new, reliable, tool increasing our knowledge of the biology of CECs and CEPs and can readily be applied in the study of many pathological conditions characterized by endothelial damage. |
| تدمد: | 1502-7686 0036-5513 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57bbba13373ef8a0f0c30e46100c3e9b https://doi.org/10.1080/00365510802673175 |
| رقم الأكسشن: | edsair.doi.dedup.....57bbba13373ef8a0f0c30e46100c3e9b |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15027686 00365513 |
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