Changes in urethral smooth muscle and external urethral sphincter function with age in rats
| العنوان: | Changes in urethral smooth muscle and external urethral sphincter function with age in rats |
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| المؤلفون: | Takuma Oshiro, Asuka Ashikari, Ryu Kimura, Minoru Miyazato, Keiichiro Izumi, Seiichi Saito |
| المصدر: | Physiological Reports, Vol 8, Iss 24, Pp n/a-n/a (2021) Physiological Reports |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | medicine.medical_specialty, detrusor underactivity, Physiology, Urinary Bladder, Urology, synergy, Electromyography, 030204 cardiovascular system & hematology, Rats, Sprague-Dawley, Masson's trichrome stain, 03 medical and health sciences, 0302 clinical medicine, Atrophy, Urethra, Physiology (medical), Urinary Bladder, Underactive, medicine, Animals, QP1-981, Urinary bladder, medicine.diagnostic_test, business.industry, Urethral sphincter, aging, Cystometry, Muscle, Smooth, Original Articles, medicine.disease, digestive system diseases, Rats, medicine.anatomical_structure, Original Article, Female, business, Perfusion, 030217 neurology & neurosurgery, Muscle Contraction |
| الوصف: | To confirm changes in urethral activity with age, both intravesical pressure and urethral perfusion pressure (UPP) were recorded and external urethral sphincter electromyography (EUS‐EMG) was performed. A total of 33 female Sprague Dawley rats aged 3 months (young rats), 12 months (middle‐aged rats), and 24 months (aged rats) were used. Bladder activity was evaluated using continuous cystometry. Urethral activity was evaluated by simultaneously recording intravesical pressure and UPP in isovolumetric conditions under urethane anesthesia in each group. Additionally, EUS‐EMG activity was monitored under the same conditions. In continuous cystometry, the amplitude of bladder contractions was not different among the three groups; nevertheless, residual urine volume was significantly increased in middle‐aged and aged rats, as compared in young rats. With respect to UPP, the change in UPP was significantly smaller in aged rats (60%) and middle‐aged rats (64%) than in young rats. Furthermore, the mean amplitude of high‐frequency oscillations of the EUS was significantly lower in aged (61%) and middle‐aged rats (70%) than in young rats. EUS‐EMG revealed EUS bursting activity during voiding with clear active and silent phases in young rats but unclear active and silent phases in aged rats. Masson's trichrome staining of the urethra showed EUS atrophy in aged rats compared to young and middle‐aged rats. The results indicate that aging induces two urethral dysfunctions in the urethral smooth muscle and EUS, which may lead to dyscoordination between the urinary bladder and urethra. To confirm changes in urethral activity with age, both intravesical pressure and urethral perfusion pressure (UPP) were recorded and external urethral sphincter electromyography (EUS‐EMG) was performed. The amplitude of bladder contractions was not different among young, middle‐aged, and aged rats; nevertheless, residual urine volume was significantly increased in middle‐aged and aged rats and the change in UPP was significantly smaller in aged rats. In histological findings, EUS hypotrophy was observed in aged rats. The results indicate that aging induces two urethral dysfunctions in the urethral smooth muscle and EUS, which may lead to dyscoordination between the urinary bladder and urethra. |
| تدمد: | 2051-817X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5810d1c3340671a83db0640df5f69d0f https://doi.org/10.14814/phy2.14643 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5810d1c3340671a83db0640df5f69d0f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2051817X |
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