Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms
| العنوان: | Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms |
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| المؤلفون: | Krešimir Pavelić, Radan Spaventi, Mladen Jokić, Sanja Kapitanović, Tamara Čačev |
| المصدر: | Journal of Cancer Research and Clinical Oncology. 132:200-204 |
| بيانات النشر: | Springer Science and Business Media LLC, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Cancer Research, Loss of Heterozygosity, Single-nucleotide polymorphism, real-time PCR, SNP, E-cadherin, Biology, Polymorphism, Single Nucleotide, law.invention, Loss of heterozygosity, law, Polymorphism (computer science), Biomarkers, Tumor, Humans, Polymerase chain reaction, Genetics, Reverse Transcriptase Polymerase Chain Reaction, General Medicine, Cadherins, Molecular biology, Real-time polymerase chain reaction, Genetic Techniques, Oncology, Genetic marker, Colonic Neoplasms, Microsatellite Repeats, SNP array |
| الوصف: | Colon cancer is a genetic disease, caused by mutations in different oncogenes and tumor-suppressor genes. The initial escape of a cancer cells from the primary tumor requires changes in cell-cell adhesion switch, which in epithelial tumors, is mediated mainly by members of the cadherin family. The aim of our study was to investigate the LOH at the E-cadherin gene locus in sporadic colon cancer as well as to evaluate the usefulness of real-time PCR SNP analysis as a new technique in this type of studies. 100 cases of human sporadic colon cancer and corresponding normal tissue samples were analyzed using 2 flanking polymorphic markers commonly used in the LOH analysis at the E-cadherin gene locus by conventional VNTR-LOH analysis. Two intragenic E-cadherin SNP markers were analyzed using real-time PCR SNP analysis. 17, 6 % of LOH was detected using flanking markers, however no LOH was detected when the intragenic E-cadherin SNP markers were introduced into our study. Since these markers are intragenic they more accurately represent the status of the E-cadherin gene than the previously used flanking markers. In conclusion, real-time PCR SNP analysis was found to be more accurate, faster, simpler and more high-throughput method than the conventional VNTR-LOH analysis. |
| تدمد: | 1432-1335 0171-5216 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::583277d846cc12d5607307b76457c8e5 https://doi.org/10.1007/s00432-005-0051-y |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....583277d846cc12d5607307b76457c8e5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14321335 01715216 |
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