Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2
| العنوان: | Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2 |
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| المؤلفون: | Shunichi Yamashita, Kazuo Umezawa, Boban Stanojevic, Biljana Vucetic-Tadic, Ana Bozovic, Lidija Todorović, Gorana Stamenković |
| المصدر: | Archives of Biological Sciences, Vol 73, Iss 1, Pp 31-38 (2021) Archives of Biological Sciences |
| بيانات النشر: | University of Belgrade, University of Novi Sad, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | NF-κB inhibitor, Chemistry, 17-Allylamino-17-demethoxygeldanamycin, targeted inhibitor, nf-κb inhibitor, synergy, Hsp90 inhibitor, General Biochemistry, Genetics and Molecular Biology, 3. Good health, Anaplastic thyroid carcinoma, Combined treatment, lcsh:Biology (General), Cell culture, Cancer research, combined treatment, General Agricultural and Biological Sciences, lcsh:QH301-705.5, hsp90 inhibitor |
| الوصف: | The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations. |
| اللغة: | English |
| تدمد: | 1821-4339 0354-4664 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5852ee4af1f13f04ec2cd259b7dcb610 http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642000055T.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5852ee4af1f13f04ec2cd259b7dcb610 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18214339 03544664 |
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