CCL21 Facilitates Chemoresistance and Cancer Stem Cell-Like Properties of Colorectal Cancer Cells through AKT/GSK-3β/Snail Signals
| العنوان: | CCL21 Facilitates Chemoresistance and Cancer Stem Cell-Like Properties of Colorectal Cancer Cells through AKT/GSK-3β/Snail Signals |
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| المؤلفون: | Hong-Sheng Wang, Yi-Fei Qi, Hao Wang, Ge Zhang, Linlin Lu, Zong-Cai Liu, Nong Wu, Shao Hui Cai, Xiao-Hui Chen, Jun Du |
| المصدر: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2016 (2016) |
| بيانات النشر: | Hindawi Publishing Corporation, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Homeobox protein NANOG, Aging, endocrine system, Article Subject, Snail, Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, biology.animal, medicine, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, lcsh:QH573-671, Protein kinase B, PI3K/AKT/mTOR pathway, Glycogen Synthase Kinase 3 beta, biology, Chemokine CCL21, lcsh:Cytology, Cancer, Cell Biology, General Medicine, medicine.disease, HCT116 Cells, Molecular biology, Up-Regulation, 030104 developmental biology, Drug Resistance, Neoplasm, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Neoplastic Stem Cells, Snail Family Transcription Factors, Stem cell, Colorectal Neoplasms, Proto-Oncogene Proteins c-akt, Research Article, Signal Transduction |
| الوصف: | Some evidence indicated that chemoresistance associates with the acquisition of cancer stem-like properties. Recent studies suggested that chemokines can promote the chemoresistance and stem cell properties in various cancer cells, while the underling mechanism is still not completely illustrated. In our study, we found that CCL21 can upregulate the expression of P-glycoprotein (P-gp) and stem cell property markers such as Bmi-1, Nanog, and OCT-4 in colorectal cancer (CRC) HCT116 cells and then improve the cell survival rate and mammosphere formation. Our results suggested that Snail was crucial for CCL21-mediated chemoresistance and cancer stem cell property in CRC cells. Further, we observed that CCL21 treatment increased the protein but not mRNA levels of Snail, which suggested that CCL21 upregulates Snail via posttranscriptional ways. The downstream signals AKT/GSK-3βmediated CCL21 induced the upregulation of Snail due to the fact that CCL21 treatment can obviously phosphorylate both AKT and GSK-3β. The inhibitor of PI3K/Akt, LY294002 significantly abolished CCL21 induced chemoresistance and mammosphere formation of HCT116 cells. Collectively, our results in the present study revealed that CCL21 can facilitate chemoresistance and stem cell property of CRC cells via the upregulation of P-gp, Bmi-1, Nanog, and OCT-4 through AKT/GSK-3β/Snail signals, which suggested a potential therapeutic approach to CRC patients. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1942-0994 1942-0900 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::588a424ddaef27d3c5ea73be504dd46d http://europepmc.org/articles/PMC4707330 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....588a424ddaef27d3c5ea73be504dd46d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19420994 19420900 |
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