Humoral response against heat shock proteins and other mycobacterial antigens after intravesical treatment with bacille Calmette–Guérin (BCG) in patients with superficial bladder cancer
العنوان: | Humoral response against heat shock proteins and other mycobacterial antigens after intravesical treatment with bacille Calmette–Guérin (BCG) in patients with superficial bladder cancer |
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المؤلفون: | K. Palfliet, M. Decock, Olivier Denis, J.P. Van Vooren, F Jurion, Alexandre R. Zlotta, H. Shekarsarai, Michel Pirson, Claude C. Schulman, Annie Drowart, Kris Huygen, Jacques Simon, J. De Bruyn, Françoise Mascart |
المصدر: | Clinical and Experimental Immunology. 109:157-165 |
بيانات النشر: | Oxford University Press (OUP), 1997. |
سنة النشر: | 1997 |
مصطلحات موضوعية: | Male, medicine.medical_treatment, Immunology, Population, Tuberculin, complex mixtures, Mycobacterium, Antigen, Antibody Specificity, Escherichia coli, Tetanus Toxoid, Humans, Immunology and Allergy, Medicine, HSP70 Heat-Shock Proteins, Lymphocytes, education, Heat-Shock Proteins, Aged, Antigens, Bacterial, education.field_of_study, biology, Immunodominant Epitopes, business.industry, Escherichia coli Proteins, Toxoid, Chaperonin 60, Original Articles, Immunotherapy, Middle Aged, Antibodies, Bacterial, Mycobacterium bovis, Recombinant Proteins, Administration, Intravesical, Immunoglobulin M, Urinary Bladder Neoplasms, Immunoglobulin G, BCG Vaccine, biology.protein, Female, Antibody, business, BCG vaccine, Cell Division, Lymphoproliferative response |
الوصف: | SUMMARY Few studies have analysed the antibody response during intravesical BCG immunotherapy for superficial bladder cancer. We have examined the evolution in serum antibody response against several heat shock proteins (hsp), including the recombinant mycobacterial hsp65 and the native protein P64 from BCG, GroEL from Escherichia coli (hsp60 family), recombinant mycobacterial hsp70 and the E. coli DnaK (hsp70 family), against purified protein derivative of tuberculin (PPD) and the AG85 complex of Mycobacterium bovis BCG, as well as against tetanus toxoid in 42 patients with a superficial bladder tumour, 28 treated with six intravesical BCG instillations and 14 patients used as controls. We also analysed the lymphoproliferative response of peripheral blood mononuclear cells against PPD in this population. Data of antibody responses at 6 weeks post BCG were available in all 28 patients, and at 4 month follow up in 17 patients. All patients who demonstrated a significant increase in IgG antibodies against PPD at 4 months follow up had a significant increase already at 6 weeks of follow up. In contrast, IgG antibodies against hsp increased significantly from 6 weeks to 4 months post-treatment. A significant increase in IgG antibodies against PPD, hsp65, P64, GroEL, and hsp70 at 4 months follow up was observed in 10/17, 8/17, 10/17, 4/17 and 8/17 patients. Native P64 protein elicited a higher antibody response than recombinant mycobacterial hsp65. No increase in antibody response was observed against Dnak from E. coli, against AG85 or tetanus toxoid after BCG therapy. An increase in IgG antibodies against P64 at 4 months follow up compared with pretreatment values was found to be a significant predictor of tumour recurrence (P |
تدمد: | 1365-2249 0009-9104 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::588aed2bc52c50b8350c00141f6bef21 https://doi.org/10.1046/j.1365-2249.1997.4141313.x |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....588aed2bc52c50b8350c00141f6bef21 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 13652249 00099104 |
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