Assembly Mechanisms of Specialized Core Particles of the Proteasome
| العنوان: | Assembly Mechanisms of Specialized Core Particles of the Proteasome |
|---|---|
| المؤلفون: | Yuko Hirano, Yuki Ohte, Hideki Yashiroda, Takeumi Kaneko, Xian Zhao, Shigeo Murata, Kazutaka Sahara, Minghui Bai |
| المصدر: | Biomolecules, Vol 4, Iss 3, Pp 662-677 (2014) Biomolecules Biomolecules; Volume 4; Issue 3; Pages: 662-677 |
| بيانات النشر: | MDPI AG, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | assembly, Proteasome Endopeptidase Complex, Protein subunit, lcsh:QR1-502, Thymus Gland, Major histocompatibility complex, Biochemistry, Article, lcsh:Microbiology, PAC3-PAC4, immunoproteasome, thymoproteasome, Protein structure, Humans, chaperone, RNA, Small Interfering, Protein Structure, Quaternary, Protein precursor, Molecular Biology, propeptide, biology, PAC1-PAC2, RNA, Cell biology, Protein Subunits, proteasome, Proteasome, UMP1, Organ Specificity, Gene Knockdown Techniques, Chaperone (protein), biology.protein, Protein Multimerization, CD8, HeLa Cells, Molecular Chaperones |
| الوصف: | The 26S proteasome has a highly complicated structure comprising the 20S core particle (CP) and the 19S regulatory particle (RP). Along with the standard CP in all eukaryotes, vertebrates have two more subtypes of CP called the immunoproteasome and the thymoproteasome. The immunoproteasome has catalytic subunits β1i, β2i, and β5i replacing β1, β2, and β5 and enhances production of major histocompatibility complex I ligands. The thymoproteasome contains thymus-specific subunit β5t in place of β5 or β5i and plays a pivotal role in positive selection of CD8+ T cells. Here we investigate the assembly pathways of the specialized CPs and show that β1i and β2i are incorporated ahead of all the other β-subunits and that both β5i and β5t can be incorporated immediately after the assembly of β3 in the absence of β4, distinct from the assembly of the standard CP in which β-subunits are incorporated in the order of β2, β3, β4, β5, β6, β1, and β7. The propeptide of β5t is a key factor for this earlier incorporation, whereas the body sequence seems to be important for the earlier incorporation of β5i. This unique feature of β5t and β5i may account for preferential assembly of the immunoproteasome and the thymoproteasome over the standard type even when both the standard and specialized subunits are co-expressed. |
| وصف الملف: | application/pdf |
| تدمد: | 2218-273X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::591675c39b4e9bf8ca1b97157828c3df https://doi.org/10.3390/biom4030662 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....591675c39b4e9bf8ca1b97157828c3df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2218273X |
|---|