Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress
العنوان: | Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress |
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المؤلفون: | Yan-Yan Wu, Cai-Lin Wang, Xue Xu, Ying Zhang, Yu-Cheng Xu, Rui-Xia Weng, Guang-Yin Xu, Yong-Chang Li, Ping-An Zhang |
المصدر: | CNS Neuroscience & Therapeutics |
بيانات النشر: | John Wiley and Sons Inc., 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | 0301 basic medicine, Male, medicine.medical_specialty, Offspring, acid‐sensitive ion channel 1, prenatal maternal stress, In situ hybridization, Neurotransmission, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Dorsal root ganglion, Pregnancy, Physiology (medical), Internal medicine, medicine, Animals, Pharmacology (medical), Patch clamp, spinal dorsal horn, Pharmacology, business.industry, Antagonist, Age Factors, Original Articles, Abdominal Pain, Rats, Up-Regulation, Blot, Acid Sensing Ion Channels, Psychiatry and Mental health, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, enterodynia, miR‐485, Spinal Cord, Prenatal Exposure Delayed Effects, Original Article, Female, business, 030217 neurology & neurosurgery, Stress, Psychological |
الوصف: | Aims Irritable bowel syndrome (IBS) is a common functional gastrointestinal disease characterized by abdominal pain. Our recent study has shown that the acid‐sensitive ion channel 1 (ASIC1) in dorsal root ganglion (DRG) is involved in stomachache of adult offspring rats subjected with prenatal maternal stress (PMS). MiR‐485 is predicted to target the expression of ASIC1. The aim of the present study was designed to determine whether miR‐485/ASIC1 signaling participates in enterodynia in the spinal dorsal horn of adult offspring rats with PMS. Methods Enterodynia was measured by colorectal distension (CRD). Western blotting, qPCR, and in situ hybridization were performed to detect the expression of ASICs and related miRNAs. Spinal synaptic transmission was also recorded by patch clamping. Results PMS offspring rats showed that spinal ASIC1 protein expression and synaptic transmission were significantly enhanced. Administration of ASICs antagonist amiloride suppressed the synaptic transmission and enterodynia. Besides, PMS induced a significant reduction in the expression of miR‐485. Upregulating the expression markedly attenuated enterodynia, reversed the increase in ASIC1 protein and synaptic transmission. Furthermore, ASIC1 and miR‐485 were co‐expressed in NeuN‐positive spinal dorsal horn neurons. Conclusions Overall, these data suggested that miR‐485 participated in enterodynia in PMS offspring, which is likely mediated by the enhanced ASIC1 activities. The present study has shown that miR‐485 negatively regulates ASIC1 expression and synaptic transmission in the spinal dorsal horn, thus eventually contributing to enterodynia of PMS offspring. It tips us to prevent the adverse effects on offspring induced by environmental stressors during pregnancy. |
اللغة: | English |
تدمد: | 1755-5949 1755-5930 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59551b5d0055dda81bc89725df916965 http://europepmc.org/articles/PMC7816206 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....59551b5d0055dda81bc89725df916965 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 17555949 17555930 |
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