Expression of human full-length MUC1 inhibits the proliferation and migration of a B16 mouse melanoma cell line
| العنوان: | Expression of human full-length MUC1 inhibits the proliferation and migration of a B16 mouse melanoma cell line |
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| المؤلفون: | Qiongshu Li, Fengli Wang, Xiaxia Sun, Fang Wang, Fei Xie, Weihua Ni, Juan Wang, Fang Fang, Sujun Gao, Guixiang Tai |
| المصدر: | Oncology Reports. 30:260-268 |
| بيانات النشر: | Spandidos Publications, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cancer Research, Cell cycle checkpoint, T cell, Cell, Active Transport, Cell Nucleus, Melanoma, Experimental, Mice, Nude, Biology, digestive system, Proto-Oncogene Proteins c-myc, Mice, Cell Movement, Cell Line, Tumor, medicine, Animals, Humans, Cyclin D1, Neoplasm Invasiveness, skin and connective tissue diseases, neoplasms, beta Catenin, Cell Proliferation, Mice, Inbred BALB C, Cell growth, Mucin-1, Cell Cycle Checkpoints, General Medicine, Cell cycle, biological factors, digestive system diseases, Cell biology, medicine.anatomical_structure, Oncology, Cell culture, Cancer research, TCF Transcription Factors, Clone (B-cell biology), A431 cells, Neoplasm Transplantation |
| الوصف: | Mucin 1 (MUC1) is a large transmembrane glycoprotein that is aberrantly overexpressed in most adenocarcinomas and certain hematological malignancies. MUC1 is known to function as an oncogene with roles in both tumor formation and progression, making it a potential target for immunotherapy. B16-MUC1 cells with human full-length MUC1 are frequently used to study the antitumor activities of MUC1-based vaccines. However, we found that the growth of B16-MUC1 cells was significantly reduced in vitro. Therefore, in this study, we established two MUC1-positive clones, B16-MUC1 9-12 and B16-MUC1 9-23, and one empty vector control clone, B16-neo, to investigate the effects of MUC1 on the cancer-related characteristics of B16 cells in vitro and in vivo. Our results demonstrated that, compared with MUC1-negative cells, cells expressing MUC1 exhibited decreased cell proliferation, increased cell cycle arrest and reduced cell migratory and invasive capacities. We further investigated several MUC1-related molecules of the β-catenin pathway, and found that the expression of MUC1 decreased the translocation of β-catenin into the nucleus, reduced the activity of T cell factor (TCF) and blocked the expression of cyclin D1 and c-Myc. Moreover, when inoculated into BALB/c nude mice, cells expressing MUC1 developed smaller tumors compared with the control cells. These results demonstrate that MUC1 expression negatively affects the malignancy of B16 cells, and suggest that the regulatory mechanisms of MUC1 as an oncoprotein are more complex than previously appreciated. |
| تدمد: | 1791-2431 1021-335X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5971ae351e867240c25868051a5cca4f https://doi.org/10.3892/or.2013.2440 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5971ae351e867240c25868051a5cca4f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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