Antioxidative, cytotoxic, and anti-metastatic potentials of Laurencia obtusa and Ulva lactuca seaweeds
| العنوان: | Antioxidative, cytotoxic, and anti-metastatic potentials of Laurencia obtusa and Ulva lactuca seaweeds |
|---|---|
| المؤلفون: | Yahya Salma, Zeina Dassouki, Reem Al Monla, Achraf Kouzayha, Hala Gali-Muhtasib, Hiba Mawlawi |
| المصدر: | Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 7, Pp 308-316 (2021) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | chemistry.chemical_classification, education.field_of_study, Antioxidant, Hydrolyzed protein, biology, Chemistry, QH301-705.5, medicine.medical_treatment, Population, RC955-962, biology.organism_classification, Biochemistry, Genetics and Molecular Biology (miscellaneous), Amino acid, HeLa, Biochemistry, Arctic medicine. Tropical medicine, medicine, Ulva lactuca, Green algae, Biology (General), Cytotoxicity, education, macroalgae, laurencia obtusa, ulva lactuca, reactive oxygen species, apoptosis |
| الوصف: | Objective: To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell lines. Methods: The antioxidant radical scavenging activity of the algal extracts was estimated using the 2,2-diphenyl-1-picrylhydrazyl colorimetric assay. Moreover, the cytotoxic potential of these bioactive extracts was studied against HCT-116 and HeLa cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay. Wound healing analysis was adopted to evaluate the anti-metastatic effects of protein extracts from both algae. Cell cycle analysis was performed by flow cytometry, and apoptotic bodies were detected using 4’,6-diamidino-2-phenylindole fluorescent staining. Amino acids of hydrolyzed protein extract were separated and identified chromatographically by high performance liquid chromatography. Results: The green algae Ulva lactuca had higher lipid content than Laurencia obtusa, whereas the latter had higher protein content with high antioxidant capacity. Protein extracts had significant dose- and time-dependent cytotoxicity and anti-metastatic activity against HCT-116 cells. Protein b extracts of both algae obtained from the chloroform:hexane solvent lipid-free residue caused morphological changes and induced apoptosis of HCT-116 cells. Further analysis revealed that apoptosis induced upon Laurencia obtusa protein b treatment was triggered via the ROS pathway, causing a significant increase in the sub-G1 population. Glycine and arginine (5.94 and 5.47 μM, respectively) were the most common amino acids in Laurencia obtusa protein b extract. Conclusions: Proteins of Laurencia obtusa and Ulva lactuca could be considered as an adjuvant nutraceutical source of pro-apoptotic and anti-metastatic biomolecules against colon cancer. |
| اللغة: | English |
| تدمد: | 2588-9222 2221-1691 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59ae5d6153e6cb40dae722128a1407f1 http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=7;spage=308;epage=316;aulast=Al |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....59ae5d6153e6cb40dae722128a1407f1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25889222 22211691 |
|---|