Effect of ozone on intestinal recovery following intestinal ischemia–reperfusion injury in a rat
| العنوان: | Effect of ozone on intestinal recovery following intestinal ischemia–reperfusion injury in a rat |
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| المؤلفون: | Bassel Haj, Igor Sukhotnik, Ron Shaoul, Yulia Pollak, Arnold G. Coran, Arie Bitterman, Ibrahim Matter |
| المصدر: | Pediatric Surgery International. 30:181-188 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Analysis of Variance, Apoptosis, Recovery of Function, General Medicine, Rats, Intestines, Rats, Sprague-Dawley, Disease Models, Animal, Intestinal Diseases, Enterocytes, Oxidants, Photochemical, Ozone, Reperfusion Injury, Pediatrics, Perinatology and Child Health, Animals, Surgery, Intestinal Mucosa, Cell Proliferation |
| الوصف: | Growing evidence suggests that ozone (O3) protects the host against pathological conditions mediated by reactive oxygen species by increasing the activity of antioxidant enzymes. The purpose of the present study was to examine the effect of O3 on intestinal recovery and enterocyte turnover after intestinal ischemia-reperfusion (IR) injury in rats.Male Sprague-Dawley rats were divided into four experimental groups: (1) sham rats underwent laparotomy; (2) sham-O3 rats underwent laparotomy and were treated with an ozone/oxygen mixture intraperitoneally and intraluminally (50 %/50 %); (3) IR rats underwent occlusion of both superior mesenteric artery and portal vein for 20 min followed by 48 h of reperfusion, and (4) IR-O3 rats underwent IR and were treated with an ozone/oxygen mixture similar to group 2. Intestinal structural changes, Park's injury score, enterocyte proliferation and enterocyte apoptosis were determined 48 h following IR. Western blot was used to determine ERK and Bax protein levels. A non-parametric Kruskal-Wallis ANOVA test was used for statistical analysis with p0.05 considered statistically significant.Treatment of IR rats with O3 resulted in a significant increase in mucosal weight in jejunum (70 %) and ileum (32 %), mucosal DNA (twofold increase) and protein (35 %) in ileum, villus height and crypt depth in jejunum (61 and 16 %, correspondingly) and ileum (31 and 43 %, correspondingly) compared to IR animals. IR-O3 rats also had a significantly lower intestinal injury score as well as a lower apoptotic index in jejunum and ileum compared and IR animals. A significant increase in cell proliferation rates in IR-O3 animals was accompanied by increased levels of p-ERK protein.Treatment with ozone prevents intestinal mucosal damage, stimulates cell proliferation and inhibits programmed cell death following intestinal IR in a rat. |
| تدمد: | 1437-9813 0179-0358 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59c9bfafbc84d7264bf248344b2aaa9b https://doi.org/10.1007/s00383-013-3448-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....59c9bfafbc84d7264bf248344b2aaa9b |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14379813 01790358 |
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