Force Spectroscopy Imaging and Constriction Assays Reveal the Effects of Graphene Oxide on the Mechanical Properties of Alginate Microcapsules
العنوان: | Force Spectroscopy Imaging and Constriction Assays Reveal the Effects of Graphene Oxide on the Mechanical Properties of Alginate Microcapsules |
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المؤلفون: | Laura Paz-Artigas, Manuel Doblaré, Albert Espona-Noguera, Clara Alcaine, Luis J. Fernández, María Virumbrales-Muñoz, Kaoutar Ziani, Jesús Ciriza, José Luis Pedraz, Laura Saenz del Burgo, Ignacio Ochoa |
المصدر: | Zaguán. Repositorio Digital de la Universidad de Zaragoza instname |
بيانات النشر: | American Chemical Society (ACS), 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | Materials science, Alginates, Graphene, Hexuronic Acids, Spectrum Analysis, technology, industry, and agriculture, Biomedical Engineering, Force spectroscopy, Oxide, Capsules, Nanotechnology, Constriction, law.invention, Biomaterials, chemistry.chemical_compound, Glucuronic Acid, chemistry, law, Graphite, Porosity |
الوصف: | Microencapsulation of cells in hydrogel-based porous matrices is an approach that has demonstrated great success in regenerative cell therapy. These microcapsules work by concealing the exogenous cells and materials in a robust biomaterial that prevents their recognition by the immune system. A vast number of formulations and additives are continuously being tested to optimize cell viability and mechanical properties of the hydrogel. Determining the effects of new microcapsule additives is a lengthy process that usually requires extensive in vitro and in vivo testing. In this paper, we developed a workflow using nanoindentation (i.e., indentation with a nanoprobe in an atomic force microscope) and a custom-built microfluidic constriction device to characterize the effect of graphene oxide (GO) on three microcapsule formulations. With our workflow, we determined that GO modifies the microcapsule stiffness and surface properties in a formulation-dependent manner. Our results also suggest, for the first time, that GO alters the conformation of the microcapsule hydrogel and its interaction with subsequent coatings. Overall, our workflow can infer the effects of new additives on microcapsule surfaces. Thus, our workflow can contribute to diminishing the time required for the validation of new microcapsule formulations and accelerate their clinical translation. |
وصف الملف: | application/pdf |
تدمد: | 2373-9878 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59da81fff37d244479f29224ea47c8e6 https://doi.org/10.1021/acsbiomaterials.0c01382 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....59da81fff37d244479f29224ea47c8e6 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 23739878 |
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