Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin‐derived peptide
| العنوان: | Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin‐derived peptide |
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| المؤلفون: | Ahmed Abdal Dayem, Ssang-Goo Cho, Sang Eun Park, Gyu Bum Yeon, Hui Gwan Goo, Seo Dong Sik, Hyun Mee Park, Dae Sung Kim, Kyung Eun Lee |
| المصدر: | Journal of Cellular Physiology. 236:3510-3520 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Scaffold, Matrigel, biology, Physiology, Clinical Biochemistry, Cell Biology, Polyvinylidene fluoride, Regenerative medicine, Embryonic stem cell, Electrospinning, Cell biology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, chemistry, 030220 oncology & carcinogenesis, biology.protein, Vitronectin, Stem cell |
| الوصف: | Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long-term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin-derived peptide-mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm-coated PVDF scaffold (VNm-PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm-PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac-related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm-PVDF scaffolds may be suitable for the long-term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine. |
| تدمد: | 1097-4652 0021-9541 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a230b5eb20f78c61d15ce98551e1b61 https://doi.org/10.1002/jcp.30095 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5a230b5eb20f78c61d15ce98551e1b61 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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