Cellular and Molecular Targets of Benzo[a]pyrene and Metal Toxicity in Xenopus laevis Embryos and in Hep G2 Cells
| العنوان: | Cellular and Molecular Targets of Benzo[a]pyrene and Metal Toxicity in Xenopus laevis Embryos and in Hep G2 Cells |
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| المؤلفون: | Chiara Urani, Marina Camatini, Patrizia Bonfanti, Anita Colombo, Silvia Crippa |
| المساهمون: | Camatini, M, Bonfanti, P, Colombo, A, Urani, C, Crippa, S |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | SAGE Publications, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | metal, Xenopus, DIOL-EPOXIDE, CYTOSKELETAL ALTERATIONS, Toxicology, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, POLYCYCLIC AROMATIC-HYDROCARBONS, chemistry.chemical_compound, Xenopus laevis embryo, medicine, HEAT-SHOCK PROTEINS, OXIDATIVE STRESS, CARCINOGEN-DNA ADDUCTS, cell target, biology, benzo[a]pyrene, IN-VITRO, General Medicine, Glutathione, biology.organism_classification, DEVELOPMENTAL TOXICITY, In vitro, Hep G2, Medical Laboratory Technology, Benzo(a)pyrene, chemistry, Biochemistry, Cell culture, COPPER IONS, Zinc toxicity, Hep G2 cell, Pyrene, METABOLIC-ACTIVATION |
| الوصف: | This paper describes the use of two in vitro systems (stage 35 Xenopus laevis embryos and the human hepatoblastoma cell line, Hep G2) to study effects of some environmental contaminants (benzo[a]pyrene, copper and zinc), which are representative of chemicals with different cell targets and mechanisms of action. The ability to activate benzo[a]pyrene and to metabolise it with the cytochrome P4501A isozyme were demonstrated in both in vitro systems by assessing the formation of water-soluble and protein-bound benzo[a]pyrene metabolites and by immunochemical analysis. In X. laevis embryos, the formation of DNA adducts demonstrated the ability to produce benzo[a]pyrene reactive metabolites. Moreover, in Hep G2 cells, the cytoskeletal protein, tubulin, and the reduced form of glutathione proved to be the cellular targets of copper and zinc toxicity. In response to metal-induced stress in Hep G2 cells, there was a cytoplasmic reorganisation of heat shock protein, Hsp 70. In conclusion, the in vitro systems provide for a rapid evaluation of heterogeneous compounds such as benzo[a]pyrene and heavy metals that differ in toxic potency and mechanisms of action. They could also be used to study the mechanisms of toxic action and to identify specific cellular and molecular targets. |
| تدمد: | 2632-3559 0261-1929 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a4ffbd4e6848328aae1b502a2521610 https://doi.org/10.1177/026119299902700321 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5a4ffbd4e6848328aae1b502a2521610 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26323559 02611929 |
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