Synchrotron radiation FTIR imaging in minutes: a first step towards real-time cell imaging
| العنوان: | Synchrotron radiation FTIR imaging in minutes: a first step towards real-time cell imaging |
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| المؤلفون: | Massimo Piccinini, M. Cestelli-Guidi, Michel Moenner, Augusto Marcelli, Cyril Petibois |
| المصدر: | Analytical and Bioanalytical Chemistry. 397:2123-2129 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Diagnostic Imaging, Chemical imaging, Chemistry, business.industry, Cells, Synchrotron radiation, Infrared spectroscopy, Biochemistry, Cellular Structures, Fourier transform spectroscopy, Cell Line, Analytical Chemistry, Optics, Spectroscopy, Fourier Transform Infrared, Microscopy, Globar, Humans, Organic Chemicals, business, Absorption (electromagnetic radiation), Image resolution, Synchrotrons |
| الوصف: | FTIR microscopy with a focal plane array (FPA) of detectors enables routine chemical imaging on individual cells in only a few minutes. The brilliance of synchrotron radiation (SR) IR sources may enhance the signal obtained from such small biosamples containing small amounts of organic matter. We investigated individual cells obtained from a cell culture specifically developed for transmission FTIR imaging using either a Globar or an SR source coupled to the same instrumentation. SR-IR source focussing was optimized to control the energy distribution on the FPA of detectors. Here we show that accessing the IR absorption distribution from all the organic contents of cells at 1 x 1 microm pixel resolution was possible only with high circulating current (or = 1.2 A) illuminating a limited number of the FPA's detectors to increase the signal-to-noise ratio of IR images. Finally, a high-current SR ring is mandatory for collecting FTIR images of biosamples with a high contrast in minutes. |
| تدمد: | 1618-2650 1618-2642 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b4119793a5804e57e3197a17acc56d5 https://doi.org/10.1007/s00216-010-3817-2 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5b4119793a5804e57e3197a17acc56d5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16182650 16182642 |
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