Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS
| العنوان: | Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS |
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| المؤلفون: | Xiaomei Yan, Ruyue Fan, Yanan Gong, Xiurui Han, Dongjie Fan, Maojun Zhang, Di Xiao, Lu Sun, Tong Wang, Yuanhai You, Lihua He, Jianzhong Zhang |
| المصدر: | BioMed Research International, Vol 2020 (2020) BioMed Research International |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Article Subject, Interactome, General Biochemistry, Genetics and Molecular Biology, law.invention, Nucleic acid metabolism, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Transcription (biology), law, Stomach Neoplasms, Tandem Mass Spectrometry, Cell Line, Tumor, Humans, Adhesins, Bacterial, Chromatography, High Pressure Liquid, General Immunology and Microbiology, biology, Chemistry, General Medicine, Helicobacter pylori, biology.organism_classification, Molecular biology, Neoplasm Proteins, 030104 developmental biology, Cell culture, 030220 oncology & carcinogenesis, Cancer cell, Recombinant DNA, Medicine, Bacterial outer membrane, Research Article, Protein Binding |
| الوصف: | HpaA as an outer membrane protein of Helicobacter pylori (H. pylori) plays a significant role in the adhesion to the human stomach, but the functional relation between HpaA and gastric epithelial cells is still not clear. To screen the interaction between HpaA and cellular proteins in gastric epithelial cells, the HpaA protein from H. pylori 26695 fused with a tag (6× His) was expressed and purified successfully, the secondary structure was estimated by the Circular Dichroism (CD) spectrum, and the purified recombinant protein was used to perform the pull-down assays with gastric cancer cell lines (AGS and SGC-7901) lysates, respectively. The pull-down proteins were identified by high-performance liquid chromatography tandem mass spectrometry system (HPLC-MS/MS). A total of 9 and 13 proteins related were analyzed from AGS and SGC-7901 cell lysates, respectively. ANXA2 was considered as putative HpaA functional partner discovered from lysates of both cell lines with high score and coverage. It is hypothesized that HpaA may be involved in the biological process of regulation of transcription and nucleic acid metabolism during the adhesion of H. pylori to human gastric epithelial cells, and HpaA-binding proteins also be used as targets for the development of antiadhesion drugs against H. pylori. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b4539ef60a98454d60ebdfef791e5bd https://doaj.org/article/514aa6e9471c446d849483bfecbbe3d5 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5b4539ef60a98454d60ebdfef791e5bd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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