أعرض في EDS

H2-dependent azoreduction by Shewanella oneidensis MR-1: involvement of secreted flavins and both [Ni–Fe] and [Fe–Fe] hydrogenases

التفاصيل البيبلوغرافية
العنوان: H2-dependent azoreduction by Shewanella oneidensis MR-1: involvement of secreted flavins and both [Ni–Fe] and [Fe–Fe] hydrogenases
المؤلفون: Arlette Kpebe, Jean Lorquin, Myriam Brugna, Sébastien Le Laz, Marc Rousset
المساهمون: Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), MEB, Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN)
المصدر: Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, 2014, Applied Microbiology and Biotechnology, 98 (6), pp.2699-2707. ⟨10.1007/s00253-013-5208-z⟩
Applied Microbiology and Biotechnology, Springer Verlag, 2014, Applied Microbiology and Biotechnology, 98 (6), pp.2699-2707. ⟨10.1007/s00253-013-5208-z⟩
بيانات النشر: HAL CCSD, 2014.
سنة النشر: 2014
مصطلحات موضوعية: Hydrogenase, Flavin mononucleotide, Amaranth, Flavin group, Applied Microbiology and Biotechnology, 03 medical and health sciences, chemistry.chemical_compound, Hyda, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Flavins, Extracellular, Shewanella oneidensis, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, MR-1, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, General Medicine, Periplasmic space, biology.organism_classification, chemistry, Biochemistry, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Biotechnology, Hydrogen, Azo dye
الوصف: International audience; In this paper, the hydrogen (H2)-dependent discoloration of azo dye amaranth by Shewanella oneidensis MR-1 was investigated. Experiments with hydrogenase-deficient strains demonstrated that periplasmic [Ni–Fe] hydrogenase (HyaB) and periplasmic [Fe–Fe] hydrogenase (HydA) are both respiratory hydrogenases of dissimilatory azoreduction in S. oneidensis MR-1. These findings suggest that HyaB and HydA can function as uptake hydrogenases that couple the oxidation of H2 to the reduction of amaranth to sustain cellular growth. This constitutes to our knowledge the first report of the involvement of [Fe-Fe] hydrogenase in a bacterial azoreduction process. Assays with respiratory inhibitors indicated that a menaquinone pool and different cytochromes were involved in the azoreduction process. High-performance liquid chromatography analysis revealed that flavin mononucleotide and riboflavin were secreted in culture supernatant by S. oneidensis MR-1 under H2-dependent conditions with concentration of 1.4 and 2.4 μmol g protein-1, respectively. These endogenous flavins were shown to significantly accelerate the reduction of amaranth at micromolar concentrations acting as electron shuttles between the cell surface and the extracellular azo dye. This work may facilitate a better understanding of the mechanisms of azoreduction by S. oneidensis MR-1 and may have practical applications for microbiological treatments of dye-polluted industrial effluents.
اللغة: English
تدمد: 0175-7598
1432-0614
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b463ddb464bfd1b53d3625bb26cd6e9
https://hal-amu.archives-ouvertes.fr/hal-01494493
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....5b463ddb464bfd1b53d3625bb26cd6e9
قاعدة البيانات: OpenAIRE
الوصف
تدمد:01757598
14320614