Protein engineering approaches for antibody fragments: directed evolution and rational design approaches
| العنوان: | Protein engineering approaches for antibody fragments: directed evolution and rational design approaches |
|---|---|
| المؤلفون: | Merve Arslan, Sibel Kalyoncu, Dilara Karadag |
| المصدر: | Volume: 43, Issue: 1 1-12 Turkish Journal of Biology |
| بيانات النشر: | The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Phage display, yeast surface display, Physiology, 0206 medical engineering, rational design, antibody fragment, 02 engineering and technology, Computational biology, Microbiology, Article, Antibody fragments, biophysical properties, 03 medical and health sciences, 0302 clinical medicine, Genetics, directed evolution, Molecular Biology, Antibody, afinity, biology, Antibody,antibody fragment,directed evolution,rational design,protein engineering,phage display,yeast surface display,affinity,biophysical properties, Rational design, protein engineering, Cell Biology, Protein engineering, Antigen binding, Directed evolution, 020601 biomedical engineering, 030220 oncology & carcinogenesis, biology.protein, phage display, General Agricultural and Biological Sciences |
| الوصف: | The number of therapeutic antibodies in preclinical, clinical, or approved phases has been increasing exponentially, mostly due to their known successes. Development of antibody engineering methods has substantially hastened the development of therapeutic antibodies. A variety of protein engineering techniques can be applied to antibodies to improve their affinity and/or biophysical properties such as solubility and stability. Antibody fragments (where all or some parts of constant regions are eliminated while the essential antigen binding region is preserved) are more suitable for protein engineering techniques because there are many in vitro screening technologies available for antibody fragments but not full-length antibodies. Improvement of biophysical characteristics is important in the early development phase because most antibodies fail at the later stage of development and this leads to loss of resources and time. Here, we review directed evolution and rational design methods to improve antibody properties. Recent developments in rational design approaches and antibody display technologies, and especially phage display, which was recently awarded the 2018 Nobel Prize, are discussed to be used in antibody research and development. |
| وصف الملف: | application/pdf |
| تدمد: | 1303-6092 1300-0152 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b4b7845c399b0f8c3513af22c005adc https://doi.org/10.3906/biy-1809-28 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5b4b7845c399b0f8c3513af22c005adc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13036092 13000152 |
|---|