An HPLC method for the determination of beta-carotene in milk samples is described. The method has many advantages over previous methods due to its rapidity, convenience and the small volumes of solvents required. Beta-Carotene was 100% recovered and the relative standard deviation of repeatability was 5.3%. The limit of quantitation was found to be about 30 ng/ml and the system was linear in the concentration range investigated, from 0.67 to 4 microgram/ml.
