Temporal expression profiles of ceramide and ceramide-related genes in wild-type and mPer1/mPer2 double knockout mice
| العنوان: | Temporal expression profiles of ceramide and ceramide-related genes in wild-type and mPer1/mPer2 double knockout mice |
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| المؤلفون: | Tack-Joong Kim, Kiho Bae, Yeo-Jin Kang, Yeong-Su Jang |
| المصدر: | Molecular Biology Reports. 39:4215-4221 |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Ceramide, Time Factors, Circadian clock, Endogeny, Biology, Ceramides, Mice, chemistry.chemical_compound, Sphingosine N-Acyltransferase, Genetics, Zeitgeber, Animals, RNA, Messenger, Circadian rhythm, Molecular Biology, Ceramide synthase, Mice, Knockout, Regulation of gene expression, Gene Expression Profiling, Period Circadian Proteins, General Medicine, Circadian Rhythm, Cell biology, Sphingomyelin Phosphodiesterase, Gene Expression Regulation, Liver, chemistry, Sphingomyelin |
| الوصف: | Most living organisms exhibit circadian rhythms in physiology and behavior. These oscillations are generated by an endogenous circadian clock and control many biological processes. Ceramide has attracted attention as a signal mediator in diverse cell processes including cell death and differentiation. The relationships between ceramide expression levels and the circadian clock have not previously been investigated. To determine if there are circadian variations in the content of ceramide, we measured ceramide concentrations in the livers of wild-type (WT) and mPer1/mPer2 double knockout (DKO) mice. The ceramide concentration in WT mice was dramatically increased at Zeitgeber Time 9 (ZT9; 9 h after lights-on time) and ZT21 but no rhythmicity in ceramide expression was seen in DKO mice. Because ceramide can be generated by the hydrolysis of sphingomyelin via sphingomyelinase (SMase), or by ceramide synthase (CerS)-mediated synthesis, we assayed the expression patterns of ceramide-related genes using real-time PCR. CerS2 expression levels showed a biphasic pattern of expression in WT mice but no rhythmicity in DKO mice. While the neutral SMase (nSMase) and acidic SMase (aSMase) mRNA in WT mice were expressed in a circadian manner, the correlation between the expression levels of these SMases with times of day was weak in DKO mice. Collectively, our findings suggest that both SMases and CerS2 mRNA expression are regulated by the presence of mPer1/mPer2 circadian clock genes in vivo, and imply that ceramide may play a vital role in circadian rhythms and physiology. |
| تدمد: | 1573-4978 0301-4851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ca98e74d2c64f1c3eaf41487a3609ed https://doi.org/10.1007/s11033-011-1207-2 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5ca98e74d2c64f1c3eaf41487a3609ed |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15734978 03014851 |
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