Prostaglandin E2 as a Regulator of Germ Cells during Ovarian Development
| العنوان: | Prostaglandin E2 as a Regulator of Germ Cells during Ovarian Development |
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| المؤلفون: | Sharon L. Eddie, Richard A. Anderson, Henry N. Jabbour, Andrew J. Childs, Rosemary A. L. Bayne, Craig S. Collins |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 94:4053-4060 |
| بيانات النشر: | The Endocrine Society, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Somatic cell, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, Biochemistry, chemistry.chemical_compound, Endocrinology, Pregnancy, Prostaglandin E2, Prostaglandin-E Synthases, media_common, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Regulation, Developmental, General Medicine, Immunohistochemistry, Activins, Intramolecular Oxidoreductases, medicine.anatomical_structure, Proto-Oncogene Proteins c-bcl-2, Female, lipids (amino acids, peptides, and proteins), Prostaglandin E, medicine.drug, Adult, medicine.medical_specialty, media_common.quotation_subject, Prostaglandin E2 receptor, Prostaglandin, Context (language use), Ovary, Biology, Dinoprostone, Andrology, Translational Highlights from Jcem, Fetus, Internal medicine, medicine, Humans, Receptors, Prostaglandin E, Molecular Biology, Ovulation, Brain-Derived Neurotrophic Factor, Biochemistry (medical), Receptors, Prostaglandin E, EP2 Subtype, chemistry, Oocytes, Myeloid Cell Leukemia Sequence 1 Protein, Receptors, Prostaglandin E, EP4 Subtype |
| الوصف: | The formation of primordial follicles occurs during fetal life yet is critical to the determination of adult female fertility. Prior to this stage, germ cells proliferate, enter meiosis, and associate with somatic cells. Growth and survival factors implicated in these processes include activin A (INHBA), the neurotrophins BDNF and NT4 (NTF5), and MCL1. The prostaglandins have pleiotrophic roles in reproduction, notably in ovulation and implantation, but there are no data regarding roles for prostaglandins in human fetal ovarian development.The aim of the study was to investigate a possible role for prostaglandin (PG) E(2) in human fetal ovary development.In vitro analysis of ovarian development between 8 and 20 wk gestation was performed.The expression patterns of PG synthesis enzymes and the PGE(2) receptors EP2 and EP4 in the ovary were assessed, and downstream effects of PGE(2) on gene expression were analyzed.Ovarian germ cells express the PG synthetic enzymes COX2 and PTGES as well as the EP2 and EP4 receptors, whereas COX1 is expressed by ovarian somatic cells. Treatment in vitro with PGE(2) increased the expression of BDNF mRNA 1.7 +/- 0.16-fold (P = 0.004); INHBA mRNA, 2.1 +/- 0.51-fold (P = 0.04); and MCL1 mRNA, 1.15 +/- 0.06-fold (P = 0.04), but not that of OCT4, DAZL, VASA, NTF5, or SMAD3.These data indicate novel roles for PGE(2) in the regulation of germ cell development in the human ovary and show that these effects may be mediated by the regulation of factors including BDNF, activin A, and MCL1. |
| تدمد: | 1945-7197 0021-972X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d4dd941cb9388d351d89a1f5895087f https://doi.org/10.1210/jc.2009-0755 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5d4dd941cb9388d351d89a1f5895087f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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